The fabrication of tissue-engineered vascular grafts to replace damaged vessels is a promising therapy for cardiovascular diseases. Endothelial remodeling in the lumen of TEVGs is critical for successful revascularization. However, the construction of well-functioning TEVGs remains a fundamental challenge. Herein, chiral hybrid scaffolds were prepared by electrospinning using D/L-phenylalanine based gelators [D(L)PHEG] and poly-ε-caprolactone (PCL). The chirality of scaffolds significantly affected the endothelial remodeling progress of TEVGs. Compared with L-phenylalanine based gelators/poly-ε-caprolactone (L/PCL) and PCL, D-phenylalanine based gelators/poly-ε-caprolactone (D/PCL) scaffolds enhanced cell adhesion, and proliferation and upregulated the expression of fibronectin-1, and vinculin. These results suggests that chiral hybrid scaffolds can promote endothelial remodeling of TEVGs by upregulating adhesion-associated protein levels. This study offers an innovative strategy for endothelial remodeling of TEVGs by fabricating chiral hybrid scaffolds, and provides new insight for the treatment of cardiovascular diseases.

制造组织工程血管移植物来替代受损血管是治疗心血管疾病的一种有前途的疗法。TEVG 腔内的内皮重塑对于成功的血运重建至关重要。然而，构建功能良好的 TEVG 仍然是一个基本挑战。在此，使用基于 D/L-苯丙氨酸的凝胶剂 [D(L)PHEG] 和聚-ε-己内酯 (PCL) 通过静电纺丝制备手性杂化支架。支架的手性显着影响TEVGs的内皮重塑进程。与基于L-苯丙氨酸的凝胶剂/聚-ε-己内酯（L/PCL）和PCL相比，基于D-苯丙氨酸的凝胶剂/聚-ε-己内酯（D/PCL）支架增强细胞粘附和增殖并上调纤连蛋白的表达-1，和长春花蛋白。这些结果表明手性杂交支架可以通过上调粘附相关蛋白水平来促进 TEVG 的内皮重塑。该研究通过制造手性混合支架为 TEVG 的内皮重塑提供了一种创新策略，并为心血管疾病的治疗提供了新的见解。