With the increasing number of underground engineering construction projects such as coal mining, tunnel, and subway, water inrush disasters occur more and more frequently. Inspired by the phenomenon of microbial mineralization and diagenesis, microbial-induced calcium carbonate precipitation (MICP) is used to repair cracks in cement-based materials, which provides a new idea to solve the problem of water inrush. To investigate the self-healing properties of microbial capsules, this paper selected epoxy resin E-51 cured by DMP-30 as the wall material and Bacillus pasteurii as the core materials for experiments. In this paper, a single-factor method was adopted to determine the optimal preparation process of microbial capsules and the oil-phase separation method to prepare the microbial capsules. The effects of various factors on the experimental results under different core-wall ratios, reaction time, reaction temperatures, and agitation rates were analyzed. Microbial capsules were analyzed by Fourier transform infrared spectroscopy and optical microscopy to explore the functional groups and features of microbial capsules. The experimental results showed that the microbial capsules achieved the best performance with a core-to-wall ratio of 1 : 3, a reaction temperature of 50°C, a reaction time of 40 min, and a stirring rate of 300 rpm. Meanwhile, we determined the spore survival rate of microbial capsules and finally studied the waterproofness, storage stability, and rupture under the pressure of microbial capsules. We concluded that microbial capsules have high-efficiency and self-healing properties.

中文翻译：

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环氧树脂包覆巴氏杆菌制备微生物胶囊的研究

随着煤矿、隧道、地铁等地下工程建设项目的增多，突水灾害的发生也越来越频繁。受微生物矿化成岩现象的启发，微生物诱导碳酸钙沉淀（MICP）用于修复水泥基材料裂缝，为解决突水问题提供了新思路。为研究微生物胶囊的自愈性能，本文选用经 DMP-30 固化的环氧树脂 E-51 作为壁材和巴氏杆菌作为实验的核心材料。本文采用单因素法确定微生物胶囊的最佳制备工艺，并采用油相分离法制备微生物胶囊。分析了不同芯壁比、反应时间、反应温度和搅拌速率下各种因素对实验结果的影响。通过傅里叶变换红外光谱和光学显微镜对微生物胶囊进行分析，以探索微生物胶囊的功能基团和​​特征。实验结果表明，微生物胶囊在核壁比为1:3、反应温度为50°C、反应时间为40 min、搅拌速度为300 rpm时性能最佳。同时，我们确定了微生物胶囊的孢子存活率，最后研究了微生物胶囊的防水性、储存稳定性和压力下的破裂情况。我们得出结论，微生物胶囊具有高效和自愈特性。