Bioorthogonal regulation of DNA circuits for smart intracellular microRNA imaging,Chemical Science

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Bioorthogonal regulation of DNA circuits for smart intracellular microRNA imaging
Chemical Science ( IF 7.6 ) Pub Date : 2021-11-08 , DOI: 10.1039/d1sc05214d
Yingying Chen ₁ , Xue Gong ₁ , Yuhui Gao ₁ , Yu Shang ₁ , Jinhua Shang ₁ , Shanshan Yu ₁ , Ruomeng Li ₁ , Shizhen He ₁ , Xiaoqing Liu _{1,

2} , Fuan Wang _{1,

2}

Affiliation

Catalytic DNA circuits represent a versatile toolbox for tracking intracellular biomarkers yet are constrained with low anti-interference capacity originating from their severe off-site activation. Herein, by introducing an unprecedented endogenous DNA repairing enzyme-powered pre-selection strategy, we develop a sequential and specific on-site activated catalytic DNA circuit for achieving the cancer cell-selective imaging of microRNA with high anti-interference capacity. Initially, the circuitry reactant is firmly caged by an elongated stabilizing duplex segment with a recognition/cleavage site of a cell-specific DNA repairing enzyme, which can prevent undesired signal leakage prior to its exposure to target cells. Then, the intrinsic DNA repairing enzyme of target cells can liberate the DNA probe for efficient intracellular microRNA imaging via the multiply guaranteed molecular recognition/activation procedures. This bioorthogonal regulated DNA circuit presents a modular and programmable amplification strategy for highly reliable assays of intracellular biomarkers, and provides a pivotal molecular toolbox for living systems.

中文翻译：

用于智能细胞内 microRNA 成像的 DNA 电路的生物正交调节

催化 DNA 电路代表了一种用于跟踪细胞内生物标志物的多功能工具箱，但由于其严重的异位激活而受到低抗干扰能力的限制。在此，通过引入前所未有的内源性 DNA 修复酶驱动的预选策略，我们开发了一种顺序和特异性的现场活化催化 DNA 电路，以实现具有高抗干扰能力的 microRNA 的癌细胞选择性成像。最初，电路反应物被一个细长的稳定双链体片段牢牢笼罩，该片段具有细胞特异性 DNA 修复酶的识别/切割位点，这可以在其暴露于靶细胞之前防止不希望的信号泄漏。然后，靶细胞的内在 DNA 修复酶可以释放 DNA 探针，进行有效的细胞内 microRNA 成像通过多重保证的分子识别/激活程序。这种生物正交调节的 DNA 电路为高度可靠的细胞内生物标志物分析提供了模块化和可编程的扩增策略，并为生命系统提供了一个关键的分子工具箱。

更新日期：2021-11-24

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