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Analysis on the abnormal chromosomal behaviour and the partial female fertility of allotriploid Lilium – ‘Triumphator’ (LLO) is not exceptional to the hypothesis of lily interploid hybridizations
Scientia Horticulturae ( IF 3.9 ) Pub Date : 2021-11-23 , DOI: 10.1016/j.scienta.2021.110746
Luomin Cui 1 , Yanni Sun 1 , Kongzhong Xiao 1 , Lin Wan 1 , Ju Zhong 1 , Yanmei Liu 1 , Qiulan Xie 1 , Shujun Zhou 1
Affiliation  

Based on previous publications, allotriploid (LLO) lily ‘Triumphator’ is seemingly exceptional to the hypothesis – five same genomes is essential for its endosperm development in interploid hybridizations of Lilium, because it could be hybridized with TTTT, OTOT, or even AA;however, no LLO × LL is used as control in these reports, and its abnormal meiosis is unknown. In order to confirm the speciality, the present research investigated ‘Triumphator’ (LLO, 2n = 3x = 36) for its microsporogenesis, female fertility and its progenies’ genome composition. The results of its microsporogenesis showed that the homologous chromosomes of two L-genomes formed bivalents; the chromosomes of O-genome formed univalents, suggesting that intergenomic recombination between L-genome and O-genome hardly occur. This was the reason for that no intergenomic recombinant chromosome was found in its progenies. Interploid hybridizaitons showed that the LLO × LL was much more successful than LLO × TT/OO, strongly suggesting that ‘Triumphator’ is not exceptional to the lily five same genomes of endosperm hypothesis because the endosperm genomes of LLO × LL, LLO × TT and LLO × OO are 5L + 2O, 4L + 2O + T and 4L + 3O, respectively, i.e. only the endosperm of LLO × LL containing 5 same genomes (5 L). The hypothesis is highly valuable to guide lily breeding.



中文翻译:

异源三倍体百合异常染色体行为和部分雌性生育能力分析——“胜利者”(LLO)对百合间倍体杂交假说并不例外

根据之前的出版物,异源三倍体 (LLO) 百合“Triumphator”似乎与该假设不同——五个相同的基因组对于百合间倍体杂交中的胚乳发育至关重要,因为它可以与 TTTT、OTOT 甚至 AA 杂交;然而, 在这些报告中没有使用 LLO × LL 作为对照,其异常减数分裂未知。为了确认专业性,本研究调查了“胜利者”(LLO,2 n  = 3 x = 36) 因为它的小孢子发生、雌性生育能力及其后代的基因组组成。其小孢子形成结果表明,两个L基因组的同源染色体形成二价体;O-基因组的染色体形成单价,表明L-基因组和O-基因组之间几乎不发生基因组间重组。这就是在其后代中未发现基因组间重组染色体的原因。Interploid hybridizaitons 表明LLO × LL 比LLO × TT/OO 成功得多,强烈表明'Triumphator' 不是例外的百合五个相同基因组的胚乳假说因为LLO×LL、LLO×TT和LLO×OO的胚乳基因组分别为5L+2O、4L+2O+T和4L+3O,即只有LLO×LL的胚乳含有5个相同的基因组(5L)。该假设对指导百合育种非常有价值。

更新日期:2021-11-23
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