Abstract

The fatality caused by the bacteria in medical field, environment and especially drinking water has always been a major issue. Therefore the need of new techniques for inactivation of bacteria and especially sterilization of waterborne pathogens always remain under research. Often, a noninfectious strain of E. coli was used as an indicator of pollution and energetic waterborne pathogens, which is dangerous for public. The sterilization mechanisms promoted from conventional methods like chemical methods, oxidation methods, irradiation methods to most nontoxic, non-chemical and advance electrical discharge method known as atmospheric pressure plasma discharge. Atmospheric pressure plasma (APP) is considered as a potential tool for skin pathologies, sterilizing agent and treatment of persistent wounds because of its antibacterial effects. Formulation of plasma sterilized liquids containing highly reactive oxidant species (ROS), ultraviolet (UV) radiations and shock waves of several mega Pascal (MPa), are always essential for medical and environmental applications. We have targeted a negative DC powered water surface barrier micro discharge for the production of ROS including \({\text{O}}{{{\text{H}}}^{\bullet }}\) radicals, ozone (O₃) and hydrogen peroxide (H₂O₂). Analytical results have been presented for water surface barrier micro-discharge in production of ROS in argon (Ar) and oxygen environments. Statistical methods have been implemented to estimate the yield rate of oxidant species dependent on input power. Spectral and chemical techniques have been used for detection of atmospheric and water dissolved reactive oxidant species. The application of water surface barrier micro-discharge was studied for the degradation of multidrug resistant water borne Escherichia coli (E. coli). The results demonstrated effective sterilization of water borne E. coli by micro-discharge. It has been observed that required input power of oxygen injected discharge is higher than “Ar” injected discharge and it further increases with rise in gas injection rates. A critical outcome of this current study is that the oxygen injected discharge produces higher concentration of ROS in comparison with argon injection, useful as antibacterial source.

中文翻译：

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注入氩氧的水面屏障微放电对比研究

摘要

细菌在医疗领域、环境尤其是饮用水中造成的死亡一直是一个重大问题。因此，对灭活细菌尤其是水传播病原体的灭菌的新技术的需求始终处于研究之中。通常，一种非传染性的大肠杆菌菌株被用作污染和高能量水传播病原体的指标，这对公众是危险的。灭菌机制从化学方法、氧化方法、辐照方法等传统方法提升到大多数无毒、非化学和先进的放电方法，即常压等离子体放电。由于其抗菌作用，常压等离子体 (APP) 被认为是皮肤病理学、消毒剂和治疗持续性伤口的潜在工具。配制含有高活性氧化剂 (ROS)、紫外线 (UV) 辐射和几兆帕 (MPa) 冲击波的等离子灭菌液体，对于医疗和环境应用始终是必不可少的。\({\text{O}}{{{\text{H}}}^{\bullet }}\)自由基、臭氧 (O ₃ ) 和过氧化氢 (H ₂ O ₂ )。已经提供了在氩 (Ar) 和氧气环境中产生 ROS 的水表面屏障微放电的分析结果。已经实施了统计方法来估计依赖于输入功率的氧化剂种类的产率。光谱和化学技术已用于检测大气和水中溶解的活性氧化剂物质。研究了水面屏障微放电在降解耐多药水性大肠杆菌( E.coli )中的应用。结果证明对水性大肠杆菌有效杀菌通过微放电。已经观察到，注入氧气的放电所需的输入功率高于“Ar”注入放电，并且随着气体注入速率的增加而进一步增加。目前这项研究的一个关键结果是，与注入氩气相比，注入氧气的放电产生更高浓度的 ROS，可用作抗菌源。