Objective

To determine whether increased endometrial B-cell lymphoma 6 (BCL6) expression is associated with live birth in a normal responder in vitro fertilization (IVF) population.

Design

Case-control study.

Setting

University-affiliated infertility center.

Patient(s)

Two groups of women undergoing IVF with preimplantation genetic testing for aneuploidy followed by warmed, single, euploid embryo transfer. Group 1 consisted of women who failed to achieve live birth, and group 2 consisted of women who achieved live birth.

Intervention(s)

None.

Main Outcome Measure(s)

Endometrial BCL6 expression measured by immunohistochemistry in endometrial tissue samples. Overexpression was defined by mean HSCORE with a cutoff of positivity of >1.4, as previously described in the literature.

Result(s)

Twenty-seven patients who achieved live birth and 23 patients who failed to achieve live birth were included. B-cell lymphoma 6 expression/HSCORE and live birth rate were not associated (Odds ratio [OR], 0.78 [0.24–2.55]). Using a cutoff of >1.4 for positivity, 8 of 23 samples were positive for BCL6 in the no live birth group, whereas 7 of 27 were positive in the live birth group. There was no significant association between BCL6 positivity and live birth (OR, 0.66 [0.19–2.21]).

Conclusion(s)

The proportion of patients with BCL6 positivity did not significantly differ between those who achieved live birth and those who did not. In the population of patients at our center, who compromise of women who respond normally to IVF stimulation, BCL6 overexpression was not associated with IVF success. Physicians implementing BCL6 testing as a diagnostic tool for clinical decision making should counsel patients that results may have limited utility in predicting IVF outcomes in this population.

中文翻译：

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B 细胞淋巴瘤 6 表达与体外受精正常反应者的活产无关

客观的

确定增加的子宫内膜 B 细胞淋巴瘤 6 (BCL6) 表达是否与正常应答体外受精 (IVF) 人群的活产有关。

设计

病例对照研究。

环境

大学附属不孕不育中心。

患者）

两组接受试管受精的妇女进行非整倍体植入前基因检测，然后进行加热的单倍体整倍体胚胎移植。第 1 组由未能实现活产的妇女组成，第 2 组由实现活产的妇女组成。

干预措施

没有任何。

主要观察指标）

子宫内膜组织样本中通过免疫组织化学测量的子宫内膜 BCL6 表达。过度表达由平均 HSCORE 定义，阳性截止值 > 1.4，如先前在文献中所述。

结果）

包括 27 名实现活产的患者和 23 名未能实现活产的患者。B 细胞淋巴瘤 6 表达/HSCORE 与活产率无关（优势比 [OR]，0.78 [0.24–2.55]）。使用大于 1.4 的阳性截止值，在无活产组中，23 个样本中有 8 个 BCL6 呈阳性，而在活产组中，27 个样本中有 7 个呈阳性。BCL6 阳性与活产之间没有显着关联（OR，0.66 [0.19–2.21]）。

结论

BCL6 阳性患者的比例在实现活产和未实现活产的患者之间没有显着差异。在我们中心的患者群体中，对 IVF 刺激反应正常的女性做出妥协，BCL6 过度表达与 IVF 成功无关。将 BCL6 检测作为临床决策诊断工具的医生应告知患者，结果在预测该人群的 IVF 结果方面可能作用有限。