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Improved extraction of bioactive compounds from Monteverdia aquifolia leaves by pressurized-liquid and ultrasound-assisted extraction: Yield and chemical composition
The Journal of Supercritical Fluids ( IF 3.9 ) Pub Date : 2021-11-19 , DOI: 10.1016/j.supflu.2021.105468
Tales Prado Alves 1 , Carina Contini Triques 1 , Paula Alessandra Palsikowski 1 , Camila da Silva 2 , Mônica Lady Fiorese 1 , Edson Antonio da Silva 1 , Marcia R. Fagundes-Klen 1
Affiliation  

Monteverdia aquifolia leaves extracts was obtained using pressurized-liquid extraction (PLE) and ultrasound-assisted extraction (UAE), evaluating, when pertinent, temperature, solvent, solvent/biomass ratio, and sonication amplitude (%), comparing to the conventional Soxhlet extraction (SOX) concerning yield (wt%), total phenolic content (TPC), antioxidant activity (AA), and chemical composition. The highest yield was 8.3% obtained in SOX with ethanol (360 min and ± 78 °C). With the same solvent, UAE reached 6.6% (30 min, 50 °C, amplitude 80%, and solvent/biomass ratio 20 mL g−1), and the PLE reached 5.3% (25 min and 60 °C), corresponding to 79.2% and 63.7% of the SOX yield, respectively. However, UAE and PLE consumed a smaller volume of solvent, operated for a shorter time, and provided improved extracts, with higher TPC and AA, compared to conventional extraction. The extracts proved to be a good source of bioactive compounds, such as Vitamin E, Friedelin, Friedelanol, and Squalene.



中文翻译:

通过加压液体和超声辅助提取改进从 Monteverdia aquifolia 叶子中提取生物活性化合物:产量和化学成分

使用加压液体提取 (PLE) 和超声辅助提取 (UAE) 获得蒙特维迪亚 (Monteverdia aquifolia)叶提取物,并在相关时评估温度、溶剂、溶剂/生物质比和超声处理幅度 (%),与传统的索氏提取相比(SOX) 关于产量 (wt%)、总酚含量 (TPC)、抗氧化活性 (AA) 和化学成分。在含乙醇的 SOX 中获得的最高产率为 8.3%(360 分钟和 ± 78 °C)。使用相同的溶剂,UAE 达到 6.6%(30 分钟,50 °C,振幅 80%,溶剂/生物质比 20 mL g -1),PLE 达到 5.3%(25 分钟和 60°C),分别对应于 SOX 产率的 79.2% 和 63.7%。然而,与传统提取相比,UAE 和 PLE 消耗的溶剂量更小,操作时间更短,并且提供了改进的提取物,具有更高的 TPC 和 AA。事实证明,这些提取物是生物活性化合物的良好来源,例如维生素 E、弗里德林、弗里德醇和角鲨烯。

更新日期:2021-11-26
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