Rural environments lack basic sanitation services. Facilities for obtaining water and disposing sewage are often under the initiative of each resident, who may not be able to build and maintain them properly. Thus, water for human consumption is subject to fecal contamination and, consequently, the presence of waterborne pathogens, such as enteric viruses. This study evaluated fecal contamination of water samples from individual sources used for domestic water supply on small farms in the state of Goiás, Brazil. Samples were collected from 78 houses whose water sources were tubular wells, dug wells, springs, and surface waters. Escherichia coli (EC) bacteria, analyzed by the defined chromogenic substrate method, was used as a traditional indicator of fecal contamination. The enteric viruses Human mastadenovirus (HAdV) and Enterovirus (EV), analyzed by qPCR, were tested as complementary indicators of fecal contamination. At least one of these markers was found in 89.7% of the samples. Detection rates were 79.5% for EC, 52.6% for HAdV, and 5.1% for EV. The average concentration for EC was 8.82 × 10¹ most probable number (MPN) per 100 mL, while for HAdV and EV the concentrations were 7.51 × 10⁵ and 1.89 × 10⁶ genomic copies (GC) per liter, respectively. EC was the most frequent marker in ground and surface water samples. HAdV was detected significantly more frequently in groundwater than in surface water and was more efficient in indicating contamination in tubular wells. There was no association of frequencies or correlation of concentrations between EC and HAdV. HAdV indicated human fecal contamination and performed well as a complementary indicator. The results reveal that a large part of the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens.

农村环境缺乏基本的卫生服务。取水和污水处理设施往往由每个居民主动提出，他们可能无法妥善建造和维护。因此，人类饮用的水会受到粪便污染，因此会存在水传播病原体，例如肠道病毒。本研究评估了巴西戈亚斯州小农场生活用水的各个来源的水样的粪便污染情况。从水源为管井、挖井、泉水和地表水的 78 间房屋中采集样本。通过定义的显色底物方法分析的大肠杆菌(EC) 细菌被用作粪便污染的传统指标。肠道病毒通过 qPCR 分析的人类乳腺病毒 (HAdV) 和肠道病毒(EV) 作为粪便污染的补充指标进行了测试。在 89.7% 的样本中至少发现了一种这些标志物。EC 的检出率为 79.5%，HAdV 为 52.6%，EV 为 5.1%。EC 的平均浓度为 8.82 × 10 ¹最可能数 (MPN) 每 100 mL，而 HAdV 和 EV 的浓度为 7.51 × 10 ⁵和 1.89 × 10 ⁶分别为每升基因组拷贝数 (GC)。EC 是地下水和地表水样品中最常见的标志物。在地下水中检测到 HAdV 的频率明显高于在地表水中，并且在指示管井中的污染方面更有效。EC和HAdV之间的频率或浓度没有关联。HAdV 表明人类粪便污染并作为补充指标表现良好。结果表明，大部分被分析的人群易患由肠道病原体引起的水传播疾病。