The community of bacteria that colonize the urinary tract, the urinary microbiome, is hypothesized to influence a wide variety of urinary tract conditions. Older adults who reside in nursing homes are frequently diagnosed and treated for urinary tract conditions such as urinary tract infection. We investigated the urinary microbiome of older adults residing in a nursing home to determine if there are features of the urinary microbiome that are associated with specific conditions and exposure in this population. We were also interested in the stability of urinary microbiome over time and in similarities between the urinary and gastrointestinal microbiome. Urine samples were prospectively collected over a period of 10 months from a cohort of 26 older adults (aged >65 years) residing in a single nursing home located in Central Massachusetts. Serial samples were obtained from 6 individuals over 10 months and 5 participants were concurrently enrolled in a study of the gastrointestinal microbiome. Information collected on participants included demographics, medical history, duration of residence in the nursing home, frailty, dementia symptoms, urinary symptoms, antibiotic treatment, urinary catheterization, and hospitalizations over a 10-month period. Clean catch, midstream urine samples were collected and stored at −80°C. DNA was extracted and 16S rRNA gene sequencing was performed. The length of stay in the nursing facility and the Clinical Frailty Scale correlated with significant changes in microbiome composition. An increase in the relative abundance of a putative urinary pathogen, Aerococcus urinae, was the largest factor influencing change that occurred over the duration of residence.

中文翻译：

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居住在疗养院的老年人的尿液微生物组随居住时间的长短而变化，并显示潜在病原体增加

定植于泌尿道的细菌群落，即泌尿道微生物组，被假设会影响多种泌尿道疾病。居住在疗养院的老年人经常被诊断和治疗尿路感染等尿路疾病。我们调查了居住在疗养院的老年人的尿液微生物组，以确定尿液微生物组的特征是否与该人群的特定条件和暴露相关。我们还对尿液微生物组随时间的稳定性以及尿液和胃肠道微生物组之间的相似性感兴趣。在 10 个月的时间里，前瞻性地从居住在位于马萨诸塞州中部的一家疗养院中的 26 名老年人（年龄＞65 岁）中收集了尿样。在 10 个月内从 6 个个体获得了系列样本，5 名参与者同时参加了一项胃肠道微生物组研究。收集的参与者信息包括人口统计学、病史、在疗养院的居住时间、虚弱、痴呆症状、泌尿系统症状、抗生素治疗、导尿和 10 个月的住院治疗。收集干净的捕获物、中流尿液样本并储存在-80°C。提取 DNA 并进行 16S rRNA 基因测序。在护理机构停留的时间长短和临床虚弱量表与微生物组组成的显着变化相关。假定的泌尿道病原体 Aerococcus urinae 的相对丰度增加，