The abundance of accumulated oil palm wastes has led to their usage as substrate in the production of organic acid such as succinic acid (SA). For the first time, oil palm empty fruit bunch (OPEFB) which was pretreated using inorganic salts was utilized for SA production in a large-scale fermenter. OPEFB was pretreated sequentially using 15% (w/v) Na₃PO₄.12H₂O and 5% (w/v) ZnCl₂ solution before undergoing fermentation process in a 16 L bioreactor in the presence of Actinobacillus succinogenes ATCC 55618. The optimum fermentation conditions were adopted from small-scale setting and implemented in the large-scale setting. Two different fermentation approaches were studied; simultaneous saccharification and fermentation (SSF) and modified simultaneous saccharification and fermentation (mSSF). This research revealed that mSSF produced higher concentration of total reducing sugars (TRS) yield and SA compared to the SSF method. SSF of inorganic salts pretreated OPEFB produced maximum SA concentration (38.85 gL^-1) and yield (0.39 gg^-1). Meanwhile highest TRS yield obtained from pre-hydrolysis was 0.79 gg^-1 at 72 h and SA (50.5 gL^-1) was acquired under the mSSF at 132 h. Therefore, mSSF is a novel process that has propitious potential for SA production in a large-scale setting which can be further executed in the industrial scale.

大量积累的油棕废料导致它们被用作生产琥珀酸 (SA) 等有机酸的底物。首次使用无机盐预处理的油棕空果串 (OPEFB) 在大型发酵罐中用于 SA 生产。OPEFB 依次使用 15% (w/v) Na ₃ PO ₄ .12H ₂ O 和 5% (w/v) ZnCl ₂溶液预处理，然后在 16 L 生物反应器中在存在琥珀酸放线杆菌的情况下进行发酵过程ATCC 55618。最佳发酵条件从小规模环境中采用，在大规模环境中实施。研究了两种不同的发酵方法；同步糖化发酵 (SSF) 和改良同步糖化发酵 (mSSF)。该研究表明，与 SSF 方法相比，mSSF 产生了更高浓度的总还原糖 (TRS) 产量和 SA。无机盐预处理的OPEFB 的SSF 产生最大SA 浓度(38.85 gL ^-1 ) 和产量(0.39 gg ^-1 )。同时，从预水解获得的最高 TRS 产量在 72 小时为 0.79 gg ^-1，SA (50.5 gL ^-1) 是在 mSSF 下在 132 小时获得的。因此，mSSF 是一种新工艺，具有在大规模环境中生产 SA 的有利潜力，可以进一步在工业规模上执行。