Parkinson’s disease (PD) is one of the most common neurodegenerative disease, and is so far not considered curable. PD patients suffer from several motor and non-motor symptoms, including gastrointestinal dysfunctions and alterations of the enteric nervous system. Constipation and additional intestinal affections can precede the classical motor symptoms by several years. Recently, we reported effects of PD and related medications on the faecal bacterial community of 34 German PD patients and 25 age-matched controls. Here, we used the same collective and analysed the V6 and V7 hypervariable region of PCR-amplified, eukaryotic 18S rRNA genes using an Illumina MiSeq platform. In all, 53% (18) of the PD samples and 72% (18) of the control samples yielded sufficient amplicons for downstream community analyses. The PD samples showed a significantly lower alpha and a different beta eukaryotic diversity than the controls. Most strikingly, we observed a significantly higher relative abundance of sequence affiliated with the Geotrichum genus in the PD samples (39.7%), when compared to the control samples (0.05%). In addition, we observed lower relative abundances of sequences affiliated with Aspergillus/Penicillium, Charophyta/Linum, unidentified Opisthokonta and three genera of minor abundant zooflagellates in the PD samples. Our data add knowledge to the small body of data about the eukaryotic microbiota of PD patients and suggest a potential association of certain gut eukaryotes and PD.

帕金森病（PD）是最常见的神经退行性疾病之一，迄今为止尚不被认为是可治愈的。 PD 患者患有多种运动和非运动症状，包括胃肠功能障碍和肠神经系统改变。便秘和其他肠道疾病可能会先于典型的运动症状几年。最近，我们报告了 PD 和相关药物对 34 名德国 PD 患者和 25 名年龄匹配对照者粪便细菌群落的影响。在这里，我们使用相同的集合，并使用 Illumina MiSeq 平台分析了 PCR 扩增的真核 18S rRNA 基因的 V6 和 V7 高变区。总的来说，53% (18) 的 PD 样本和 72% (18) 的对照样本产生了足够的扩增子用于下游群落分析。 PD 样本显示出明显低于对照的 α 和不同的 β 真核生物多样性。最引人注目的是，与对照样本 (0.05%) 相比，我们观察到 PD 样本中地霉属序列的相对丰度显着更高 (39.7%)。此外，我们在 PD 样品中观察到与曲霉属/青霉属、轮藻门/亚麻属、未鉴定的后康塔属和三个属次丰富的动物鞭毛虫属相关的序列的相对丰度较低。我们的数据为有关帕金森病患者真核微生物群的少量数据增添了知识，并表明某些肠道真核生物与帕金森病之间存在潜在关联。