Abstract

Fluconazole-sensitive (CaS) and -resistant (CaR) C. albicans were grown as single-species and dual-species biofilms with Lactobacillus casei (Lc) and Lactobacillus rhamnosus (Lr). Single-species Lc and Lr were also evaluated. Biofilm analysis included viable plate counts, the extracellular matrix components, biomass, and structural organization. Lc reduced the viability of CaS, water-soluble polysaccharides, and eDNA in CaS + Lc biofilm. Lc biofilm presented more eDNA than CaS. The total biomass of CaS + Lc biofilm was higher than the single-species biofilms. The viability of Lc and Lr was reduced by CaR dual-species biofilms. The total and insoluble biomass in CaS + Lr was higher than in single-species CaS biofilms. Lc hindered the growth of CaS, and their association hampered matrix components linked to the structural integrity of the biofilm. These findings allow understanding of how the implementation of probiotics influences the growth of C. albicans biofilms and thereby helps with the development of novel approaches to control these biofilms.

摘要

氟康唑敏感 (CaS) 和耐药 (CaR)白色念珠菌生长为具有干酪乳杆菌(Lc) 和鼠李糖乳杆菌的单物种和双物种生物膜（左）。还评估了单种 Lc 和 Lr。生物膜分析包括活板计数、细胞外基质成分、生物量和结构组织。Lc 降低了 CaS + Lc 生物膜中 CaS、水溶性多糖和 eDNA 的活力。Lc 生物膜比 CaS 呈现更多的 eDNA。CaS + Lc生物膜的总生物量高于单种生物膜。CaR 双物种生物膜降低了 Lc 和 Lr 的活力。CaS + Lr 中的总生物量和不溶性生物量高于单一种类的 CaS 生物膜。Lc 阻碍了 CaS 的生长，它们的结合阻碍了与生物膜结构完整性相关的基质成分。这些发现有助于了解益生菌的使用如何影响白色念珠菌的生长 生物膜，从而有助于开发控制这些生物膜的新方法。