Abstract

This study evaluated the effect of sodium hexametaphosphate (HMP), administered alone or in combination with fluoride (F), on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were treated with HMP solutions at 0.25%, 0.5% and 1%, alone or combined with F (0.05%), and compared by evaluating their structure and quantifying the colony-forming units (CFUs), metabolic activity, production of biomass and extracellular matrix components. All HMP-containing solutions were capable of reducing metabolic activity, the biofilm biomass, and the extracellular matrix components. Furthermore, the treatment with 1% HMP/F significantly reduced the CFUs of S. mutans, although it showed no effect on the CFUs of C. albicans, in the dual-species biofilms. In general, the combination of HMP and F influenced all the parameters analyzed from dual-species biofilms, except the CFUs of C. albicans.

摘要

本研究评估了六偏磷酸钠 (HMP) 单独给药或与氟化物 (F) 联合给药对变形链球菌和白色念珠菌双物种生物膜的影响。生物膜分别用 0.25%、0.5% 和 1% 的 HMP 溶液单独或与 F（0.05%）组合处理，并通过评估它们的结构和量化菌落形成单位（CFU）、代谢活性、生物质的产生和细胞外基质成分。所有含有 HMP 的溶液都能够降低代谢活性、生物膜生物量和细胞外基质成分。此外，1% HMP/F 处理显着降低了变形链球菌的 CFU，尽管它对白色念珠菌的 CFU 没有影响，在双物种生物膜中。一般来说，HMP 和 F 的组合影响了从双物种生物膜分析的所有参数，除了白色念珠菌的 CFU 。