Selected Mechanistic Aspects of Viral Inactivation by Peracetic Acid,Environmental Science & Technology

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Selected Mechanistic Aspects of Viral Inactivation by Peracetic Acid
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-11-18 , DOI: 10.1021/acs.est.1c04302
Bradley W Schmitz ₁ , Hanwei Wang ₁ , Kellogg Schwab ₁ , Joseph Jacangelo _{1,

2}

Affiliation

Peracetic acid (PAA) is an alternative to traditional wastewater disinfection as it has a high oxidation potential without producing chlorinated disinfection byproducts. Reports have shown the effectiveness of PAA to reduce waterborne viruses, but the mechanism of inactivation is understudied. This study evaluated PAA consumption by amino acids and nucleotides that are the building blocks of both viral capsids and genomes. Cysteine (>1.7 min^–1) and methionine (>1.2 min^–1) rapidly consumed PAA, while cystine (1.9 × 10^–2 min^–1) and tryptophan (1.4 × 10^–4 min^–1) reactions occurred at a slower rate. All other amino acids and nucleotides did not react significantly (p < 0.05) with PAA during experiments. Also, PAA treatment did not result in significant (p < 0.05) reductions of purified RNA from MS2 bacteriophage and murine norovirus. Data in this study suggest that PAA effectively inactivates viruses by targeting susceptible amino acids on capsid proteins and does not readily damage viral genomes. Knowledge of virus capsid structures and protein compositions can be used to qualitatively predict the relative resistance or susceptibility of virus types to PAA. Capsid structures containing a higher total number of target amino acids may be more susceptible to PAA reactions that damage structural integrity resulting in inactivation.

中文翻译：

过氧乙酸灭活病毒的机制选择

过氧乙酸 (PAA) 是传统废水消毒的替代品，因为它具有很高的氧化潜力，而且不会产生氯化消毒副产物。报告显示 PAA 可有效减少水传播病毒，但其灭活机制尚未得到充分研究。这项研究评估了氨基酸和核苷酸对 PAA 的消耗，这些氨基酸和核苷酸是病毒衣壳和基因组的组成部分。半胱氨酸 (>1.7 min ^–1 ) 和蛋氨酸 (>1.2 min ^–1 ) 迅速消耗 PAA，而胱氨酸 (1.9 × 10 ^–2 min ^–1 ) 和色氨酸 (1.4 × 10 ^–4 min ^–1 ) 反应发生较慢速度。所有其他氨基酸和核苷酸没有显着反应（p< 0.05) 在实验过程中使用 PAA。此外，PAA 处理并未导致来自 MS2 噬菌体和鼠诺如病毒的纯化 RNA 的显着减少（p < 0.05）。本研究中的数据表明，PAA 通过靶向衣壳蛋白上的易感氨基酸来有效灭活病毒，并且不易损坏病毒基因组。病毒衣壳结构和蛋白质组成的知识可用于定性预测病毒类型对 PAA 的相对抗性或易感性。含有更多目标氨基酸总数的衣壳结构可能更容易受到 PAA 反应的影响，PAA 反应会破坏结构完整性，从而导致失活。

更新日期：2021-12-07

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