T Lymphocyte-Captured DNA Network for Localized Immunotherapy,Journal of the American Chemical Society

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T Lymphocyte-Captured DNA Network for Localized Immunotherapy
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2021-11-15 , DOI: 10.1021/jacs.1c07036
Chi Yao ₁ , Chenxu Zhu ₁ , Jianpu Tang ₁ , Junhan Ou ₁ , Rui Zhang ₁ , Dayong Yang ₁

Affiliation

The efficient isolation of immune cells with high purity and low cell damage is important for immunotherapy and remains highly challenging. We herein report a cell capture DNA network containing polyvalent multimodules for the specific isolation and in situ incubation of T lymphocytes (T-cells). Two ultralong DNA chains synthesized by an enzymatic amplification process were rationally designed to include functional multimodules as cell anchors and immune adjuvants. Mutually complementary sequences facilitated the formation of a DNA network and encapsulation of T-cells, as well as offering cutting sites of a restriction enzyme for the responsive release of T-cells and immune adjuvants. The purity of captured tumor-infiltrating T-cells reached 98%, and the viability of T-cells maintained ∼90%. The T-cells-containing DNA network was further administrated to a tumor lesion for localized immunotherapy. Our work provides a robust nanobiotechnology for efficient isolation of immune cells and other biological particles.

中文翻译：

用于局部免疫治疗的 T 淋巴细胞捕获的 DNA 网络

高效分离具有高纯度和低细胞损伤的免疫细胞对于免疫治疗非常重要，并且仍然具有很高的挑战性。我们在此报告了一种包含多价多模块的细胞捕获 DNA 网络，用于特定的分离和原位T淋巴细胞（T细胞）的孵育。通过酶促扩增过程合成的两条超长 DNA 链经过合理设计，包括作为细胞锚和免疫佐剂的功能性多模块。相互互补的序列促进了 DNA 网络的形成和 T 细胞的封装，并为 T 细胞和免疫佐剂的响应释放提供了限制性内切酶的切割位点。捕获的肿瘤浸润性 T 细胞纯度达到 98%，T 细胞的活力维持在 90% 左右。将含有 T 细胞的 DNA 网络进一步施用于肿瘤病灶以进行局部免疫治疗。我们的工作为有效分离免疫细胞和其他生物颗粒提供了强大的纳米生物技术。

更新日期：2021-11-24

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