Microfluidic fabrication of hydrogel microparticles with MOF-armoured multi-enzymes for cascade biocatalytic reactions,Reaction Chemistry & Engineering

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Microfluidic fabrication of hydrogel microparticles with MOF-armoured multi-enzymes for cascade biocatalytic reactions
Reaction Chemistry & Engineering ( IF 3.4 ) Pub Date : 2021-11-04 , DOI: 10.1039/d1re00257k
Yan Zhang ₁ , Bi-Cong Wang ₁ , Po Wang ₁ , Xiao-Jie Ju _{1,

2} , Mao-Jie Zhang ₃ , Rui Xie _{1,

2} , Zhuang Liu _{1,

2} , Wei Wang _{1,

2} , Liang-Yin Chu _{1,

2}

Affiliation

Uniform hydrogel microparticles with MOF nanoparticles for molecular co-confinement of cascade enzymes are developed by droplet microfluidics to achieve enhanced stability and reusability under harsh conditions. Water-swollen polyacrylamide hydrogel is used for constructing the microparticles; while microporous zeolitic imidazolate framework-8 (ZIF-8) nanoparticles are incorporated in the hydrogel networks for multi-enzyme co-confinement. With the ZIF-8 nanoparticles as armour, the multiple enzymes in the microparticles show good stability for efficient biocatalytic cascades under harsh conditions. This is demonstrated by using glucose oxidase (GOx) and horseradish peroxidase (HRP) as typical cascade enzymes. The hydrogel microparticles with both GOx and HRP confined in their ZIF-8 nanoparticles exhibit enhanced stability for efficient biocatalytic cascades and storage, after treatment under harsh conditions involving high temperature, UV light, and protease. Moreover, the hydrogel microparticles allow easy recycling and good reusability after repeated treatment at high temperature (80 °C). This work provides a simple and efficient strategy to create multiple-enzyme-loaded microcarriers with good stability and reusability for biocatalytic cascades.

中文翻译：

用于级联生物催化反应的具有 MOF 铠装多酶的水凝胶微粒的微流体制造

通过液滴微流体技术开发了具有 MOF 纳米粒子的均匀水凝胶微粒，用于级联酶的分子共限制，以在恶劣条件下提高稳定性和可重复使用性。水溶胀聚丙烯酰胺水凝胶用于构建微粒；而微孔沸石咪唑酯骨架-8（ZIF-8）纳米颗粒则被纳入水凝胶网络中以实现多酶共限制。以 ZIF-8 纳米粒子作为盔甲，微粒中的多种酶在恶劣条件下对高效的生物催化级联反应显示出良好的稳定性。这可以通过使用葡萄糖氧化酶 (GOx) 和辣根过氧化物酶 (HRP) 作为典型的级联酶来证明。在高温、紫外线和蛋白酶等苛刻条件下处理后，将 GOx 和 HRP 限制在其 ZIF-8 纳米颗粒中的水凝胶微粒表现出增强的稳定性，可实现高效的生物催化级联和储存。此外，水凝胶微粒在高温（80°C）下反复处理后易于回收和良好的可重复使用性。这项工作提供了一种简单有效的策略来创建具有良好稳定性和生物催化级联可重复使用性的多酶负载微载体。

更新日期：2021-11-16

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