Salmonella contamination is a critical problem in poultry farms, with serious consequences for both animals and food products. The aim of this study is to investigate the use of phage cocktails to reduce Salmonella contamination in poultry farms. Within the scope of the study, Salmonella phages were isolated from chicken stool. After the host range of phages was determined, morphological characterization was performed through transmission electron microscopy analysis. Then, replication parameters and adsorption rates were determined by one-step growth curves. After that, phage cocktail was prepared, and its effectiveness was tested in three environments, which were drinking water, shavings, and plastic surfaces. The results obtained have demonstrated that the phage cocktail can reduce Salmonella count up to 2.80 log₁₀ units in drinking water, up to 2.30 log₁₀ units on shavings, and 2.31 log₁₀ units on plastic surfaces. It has been determined that phage cocktails could be a successful alternative in reducing Salmonella contamination in poultry environment. This work is the first study to investigate the use of phage cocktails for reducing Salmonella contamination in poultry water and on shavings, and it is presumed that the results obtained will contribute to the fight against pathogens by making them applicable to poultry farms.

沙门氏菌污染是家禽养殖场的一个严重问题，对动物和食品都造成严重后果。本研究的目的是调查使用噬菌体混合物减少家禽养殖场的沙门氏菌污染。在研究范围内，沙门氏菌从鸡粪中分离出噬菌体。在确定噬菌体的宿主范围后，通过透射电子显微镜分析进行形态学表征。然后，通过一步生长曲线确定复制参数和吸附率。之后，制备噬菌体混合物，并在饮用水、刨花和塑料表面三种环境中测试其有效性。获得的结果表明，噬菌体混合物可以将饮用水中的沙门氏菌计数减少多达 2.80 log _{10单位，刨花上的沙门氏菌数量减少多达 2.30 log 10}单位，塑料表面上的沙门氏菌数量减少2.31 log _10单位。已经确定，噬菌体鸡尾酒可能是减少家禽环境中的沙门氏菌污染。这项工作是第一项研究使用噬菌体混合物减少家禽水和刨花中沙门氏菌污染的研究，据推测，所获得的结果将通过使其适用于家禽养殖场而有助于对抗病原体。