Trimethylolpropane triesters – TMPTEs (biolubricants) have been produced through enzymatic hydroesterification of refined soybean (RSO) or used soybean cooking (USCO) oils. In the first step, enzymatic hydrolysis has been performed using a crude free lipase extract from Candida rugosa (CRL) to produce free fatty acids (FFAs). Complete hydrolysis of the oils was achieved after 3 h of reaction. Then, CRL and the lipases from Thermomyces lanuginosus (Lipolase® 100L and Eversa® Transform 2.0) and Pseudomonas fluorescens (Amano AK) were immobilized via interfacial activation on polystyrene-divinylbenzene (PSty-DVB) beads. High enzyme loaded biocatalysts (immobilization yield above 85%) were obtained for all lipase preparations using an initial protein loading of 40 mg g⁻¹. Then, free lipases (liquid or powder forms) or immobilized biocatalysts were employed to produce TMPTEs via enzymatic esterification of the produced FFAs with trimethylolpropane (TMP) in eco-friendly processes (solvent-free systems). The new heterogeneous biocatalyst prepared in this study (immobilized Eversa® Transform 2.0) was the most active biocatalyst in TMPTEs production and maximum OH conversion of ≈97% using both FFA sources was achieved after 4 h of reaction conducted in open reactors at 55 °C, TMP:FFA ratio of 1:3.25, 240 rpm, and 15% of mass of biocatalyst per mass of starting materials. Under similar conditions, OH conversion of ∼25% after 5 h of reaction was obtained using Novozym® 435 as biocatalyst. Immobilized Eversa® Transform 2.0 retained above 90% of its original activity after 13 consecutive reaction cycles. The produced TMPTEs presented good cold temperature properties (pour point values between −11 and −9 °C).

三羟甲基丙烷三酯 - TMPTE（生物润滑剂）是通过精制大豆 (RSO) 或用过的大豆烹饪油 (USCO) 的酶促加氢酯化生产的。在第一步中，使用来自假丝酵母(CRL)的粗游离脂肪酶提取物进行酶水解，以产生游离脂肪酸 (FFA)。反应3小时后油完全水解。然后，CRL 和来自疏毛嗜热丝孢菌（Lipolase® 100L 和 Eversa® Transform 2.0）和荧光假单胞菌的脂肪酶(Amano AK) 通过界面活化固定在聚苯乙烯-二乙烯基苯 (PSty-DVB) 珠上。使用 40 mg g ^-1的初始蛋白质负载量获得了所有脂肪酶制剂的高酶负载生物催化剂（固定化产率高于 85%）. 然后，使用游离脂肪酶（液体或粉末形式）或固定化生物催化剂通过在环保工艺（无溶剂系统）中用三羟甲基丙烷 (TMP) 酶促酯化生产的 FFA 来生产 TMPTE。本研究中制备的新型多相生物催化剂（固定化 Eversa® Transform 2.0）是 TMPTE 生产中最活跃的生物催化剂，在 55 °C 下在开放式反应器中进行 4 小时反应后，使用两种 FFA 源均实现了约 97% 的最大 OH 转化率, TMP:FFA 比率为 1:3.25，240 rpm，每质量起始材料的生物催化剂质量为 15%。在类似条件下，使用 Novozym® 435 作为生物催化剂，反应 5 小时后的 OH 转化率约为 25%。固定化 Eversa® Transform 2.0 在连续 13 个反应循环后仍保持其原始活性的 90% 以上。