ABSTRACT

Purpose

The aim of this study was to characterize the regulatory role of microRNA-29a (miR-29a) in myopia, providing support for potential biomarkers and new therapeutic targets of myopia in humans.

Methods

The miR-29a expression level was detected in the aqueous humor and peripheral blood plasma of 21 high myopic patients and eight cataract control patients using quantitative polymerase chain reaction. iTRAQ analysis of proteomes was conducted to show the regulatory effect of miR-29a on human scleral fibroblasts (SFs) cultured in vitro. We also assessed proliferation, migration, and collagen I synthesis in SF cells, mediated by miR-29a.

Results

MiR-29a expression was significantly higher in the aqueous humor of highly myopic patients than in the cataract control patients (fold change: 4.861, p = 0.001). miR-29a inhibited the synthesis of type I collagen in human SF cells and enhanced cell migration, but had no significant effect on cell proliferation.

Conclusion

MiR-29a was highly expressed in aqueous humor of myopia patients and inhibited the synthesis of type I collagen in human SF cells in vivo, thereby it may play an important role in myopia development.

中文翻译：

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MicroRNA-29a 可能通过调节胶原蛋白 I 影响近视发展

摘要

目的

本研究的目的是描述 microRNA-29a (miR-29a) 在近视中的调节作用，为人类近视的潜在生物标志物和新的治疗靶点提供支持。

方法

采用定量聚合酶链反应检测21名高度近视患者和8名白内障对照患者的房水和外周血浆中miR-29a的表达水平。进行蛋白质组的 iTRAQ 分析以显示 miR-29a 对体外培养的人巩膜成纤维细胞 (SFs) 的调节作用。我们还评估了由 miR-29a 介导的 SF 细胞中的增殖、迁移和胶原蛋白 I 合成。

结果

高度近视患者房水中的 MiR-29a 表达显着高于白内障对照患者（倍数变化：4.861，p = 0.001）。miR-29a抑制人SF细胞中I型胶原蛋白的合成并增强细胞迁移，但对细胞增殖没有显着影响。

结论

MiR-29a在近视患者房水中高表达，在体内抑制人SF细胞合成I型胶原，可能在近视发展中发挥重要作用。