Redox-Activated Contrast-Enhanced T1-Weighted Imaging Visualizes Glutathione-Mediated Biotransformation Dynamics in the Liver,ACS Nano

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Redox-Activated Contrast-Enhanced T1-Weighted Imaging Visualizes Glutathione-Mediated Biotransformation Dynamics in the Liver
ACS Nano ( IF 15.8 ) Pub Date : 2021-11-09 , DOI: 10.1021/acsnano.1c06026
Kun Liu ₁ , Bilun Kang ₁ , Xiangjie Luo ₁ , Zhaoxuan Yang ₁ , Chengjie Sun ₁ , Ao Li ₁ , Yifan Fan ₁ , Xiaoyuan Chen ₂ , Jinhao Gao ₁ , Hongyu Lin ₁

Affiliation

GSH-mediated liver biotransformation is a crucial physiological process demanding efficient research tools. Here, we report a type of amorphous Fe_xMn_yO nanoparticles (AFMO-ZDS NPs) as redox-activated probes for in vivo visualization of the dynamics of GSH-mediated biotransformation in liver with T₁-weighted magnetic resonance imaging (MRI). This imaging technique reveals the periodic variations in GSH concentration during the degradation of AFMO-ZDS NPs due to the limited transportation capacity of GSH carriers in the course of GSH efflux from hepatocytes to perisinusoidal space, providing direct imaging evidence for this important carrier-mediated process during GSH-mediated biotransformation. Therefore, this technique offers an effective method for in-depth investigations of GSH-related biological processes in liver under various conditions as well as a feasible means for the real-time assessment of liver functions, which is highly desirable for early diagnosis of liver diseases and prompt a toxicity evaluation of pharmaceuticals.

中文翻译：

氧化还原激活对比增强 T1 加权成像可视化肝脏中谷胱甘肽介导的生物转化动力学

GSH 介导的肝脏生物转化是一个重要的生理过程，需要有效的研究工具。在这里，我们报告了一种非晶态 Fe _x Mn _y O 纳米粒子 (AFMO-ZDS NPs) 作为氧化还原激活的探针，用于体内可视化 GSH 介导的肝脏生物转化动力学的T ₁-加权磁共振成像（MRI）。这种成像技术揭示了 AFMO-ZDS NPs 降解过程中 GSH 浓度的周期性变化，这是由于 GSH 载体在 GSH 从肝细胞流出到窦周空间的过程中的运输能力有限，为这一重要的载体介导过程提供了直接的成像证据在 GSH 介导的生物转化过程中。因此，该技术为深入研究肝脏在不同条件下与GSH相关的生物学过程提供了一种有效的方法，为实时评估肝功能提供了一种可行的手段，对肝病的早期诊断具有重要意义。并提示对药物进行毒性评价。

更新日期：2021-11-23

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