Countries' genetic resources and biological variation are extremely valuable. Biodiversity and genetic resources, particularly for endemic species, should be protected. DNA barcoding studies are a useful for identifying unknown taxa and protecting a country's biodiversity. DNA barcodes are small segments of DNA that are taxonomically informative and can be used to for species identification. DNA barcodes have been used to associate life phases, determine cryptic species, and prohibit illegal commerce, among other things. Astragalus argaeus and A. stenosemioides are endemic plant species to Turkey's Mt. Erciyes. To distinguish these two endemic Astagalus, we used two DNA barcodes in the matK region. The current study used A. argaeus, A. stenosemioides, and 23 GenBank sequences of other relative in the genus Astragalus, including one from a closely related species, to infer phylogenetic relationships and DNA barcodes for two endemic plants. The matK gene region could clearly identify A. argaeus and A. stenosemioides from closely related taxa, according to the phylogenetic study. Taxonomic and biodiversity research, as well as molecular ecology and population genetics investigations, will benefit from DNA barcoding surveys to unambiguously differentiate between these two species. Also, by separating the matK DNA gene area, which is one of the molecular features, it is feasible to detect the species, and A. argaeus and A. stenosemioides were successfully barcoded for the first time.

各国的遗传资源和生物变异极其宝贵。生物多样性和遗传资源，特别是特有物种，应受到保护。DNA 条形码研究有助于识别未知的分类群和保护一个国家的生物多样性。DNA 条形码是 DNA 的小片段，具有分类学信息，可用于物种识别。DNA 条形码已被用于关联生命阶段、确定神秘物种和禁止非法贸易等。黄芪和A. stenoseemioides是土耳其Erciyes 山的特有植物。为了区分这两种地方性黄芪，我们在matK区域使用了两个 DNA 条形码。目前的研究使用A. argaeus、A. stenosemioides和 23 个黄芪属中其他近缘种的 GenBank 序列，包括一个来自密切相关物种的序列，以推断两种特有植物的系统发育关系和 DNA 条形码。该matK序列基因区域可以清楚地识别A. argaeus和A. stenosemioides从密切相关的类群，根据系统发育研究。分类学和生物多样性研究，以及分子生态学和种群遗传学调查，将受益于 DNA 条形码调查，以明确区分这两个物种。此外，通过分离matK分子特征之一的DNA基因区具有检测物种的可行性，并首次成功对A. argaeus和A. stenosemioides进行条码化。