Surgery, chemotherapy and radiotherapy, the conventional treatment modalities of cancer though successful are limited by presence of residual tumor cells, toxic side-effects and treatment resistance, thus raising the need for investigating other novel approaches. Here, we have used a cold atmospheric plasma (CAP) jet and assessed the in vitro efficacy in gingivobuccal squamous cell carcinoma (GB-SCC) – ITOC-03, breast adenocarcinoma—MCF7 and HEK293 cells. Cells lines were subjected to varying doses of ionizing radiation (0, 2, 4, 6, 8 Gy) and CAP jet treatment (0, 60, 180, 240, 300 s). CAP jet treatment showed time dependent increase in H₂O₂ and NO₂⁻ concentration. Cell viability assay showed potent effect of CAP jet on all three cell lines in comparison to radiation treatment, while helium gas treatment showed minimal inhibitory effect. Irradiated, CAP jet and helium gas treated cells showed loss of nucleic acid features, 788 cm⁻¹ and 1340 cm⁻¹ in Raman spectra, indicating DNA damage. Principal Component Analysis (PCA) showed distinct classification of CAP-treated and control cells, while Principal Component – Linear Discriminant Analysis (PC-LDA) based classification of Raman spectra showed ITOC-03 and HEK293 cells to be sensitive to CAP jet and radiation treatment in comparison to MCF7 cells. Collectively, cell viability assay and Raman spectroscopy have shown potent effect of CAP jet in GB-SCC and breast adenocarcinoma cells.

手术、化学疗法和放射疗法这些癌症的常规治疗方式虽然成功，但受到残留肿瘤细胞、毒副作用和治疗耐药性的限制，因此需要研究其他新方法。在这里，我们使用冷大气等离子体 (CAP) 射流并评估了在牙龈颊鳞状细胞癌 (GB-SCC) – ITOC-03、乳腺腺癌 – MCF7 和 HEK293 细胞中的体外疗效。细胞系受到不同剂量的电离辐射（0、2、4、6、8 Gy）和 CAP 喷射处理（0、60、180、240、300 秒）。CAP 喷射处理显示 H ₂ O ₂和 NO ₂随时间增加^-专注。细胞活力测定表明，与放射治疗相比，CAP jet 对所有三种细胞系的有效作用，而氦气处理显示出最小的抑制作用。经辐照、CAP 射流和氦气处理的细胞显示出核酸特征丢失，在拉曼光谱中为788 cm ^-1和 1340 cm ^-1，表明 DNA 损伤。主成分分析 (PCA) 显示出 CAP 处理和对照细胞的不同分类，而基于主成分 – 线性判别分析 (PC-LDA) 的拉曼光谱分类显示 ITOC-03 和 HEK293 细胞对 CAP 喷射和辐射处理敏感与 MCF7 细胞相比。总的来说，细胞活力测定和拉曼光谱已显示 CAP jet 在 GB-SCC 和乳腺癌细胞中的有效作用。