Carbon Nanotube Field-Effect Transistor Biosensor for Ultrasensitive and Label-Free Detection of Breast Cancer Exosomal miRNA21,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Carbon Nanotube Field-Effect Transistor Biosensor for Ultrasensitive and Label-Free Detection of Breast Cancer Exosomal miRNA21
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-11-08 , DOI: 10.1021/acs.analchem.1c03573
Tingxian Li ₁ , Yuqi Liang ₂ , Jiahao Li ₁ , Yi Yu ₁ , Meng-Meng Xiao ₂ , Wei Ni ₃ , Zhiyong Zhang ₄ , Guo-Jun Zhang ₁

Affiliation

Tumor-derived exosomal miRNAs may have important functions in the onset and progression of cancers and are potential biomarkers for early diagnosis and prognosis monitoring. Yet, simple, sensitive, and label-free detection of exosomal miRNAs remains challenging. Herein, an ultrasensitive, label-free, and stable field-effect transistor (FET) biosensor based on a polymer-sorted high-purity semiconducting carbon nanotube (CNT) film is reported to detect exosomal miRNA. Different from conventional CNT FETs, the CNT FET biosensors employed a floating gate structure using an ultrathin Y₂O₃ as an insulating layer, and assembled Au nanoparticles (AuNPs) on Y₂O₃ as linkers to anchor probe molecules. A thiolated oligonucleotide probe was immobilized on the AuNP surface of the sensing area, after which miRNA21 was detectable by monitoring the current change before and after hybridization between the immobilized DNA probe and target miRNA. This method achieved both high sensitivity (LOD: 0.87 aM) and high specificity. Furthermore, the FET biosensor was employed to test clinical plasma samples, showing significant differences between healthy people and breast cancer patients. The CNT FET biosensor shows the potential applications in the clinical diagnosis of breast cancer.

中文翻译：

用于超灵敏和无标记检测乳腺癌外泌体 miRNA21 的碳纳米管场效应晶体管生物传感器

肿瘤来源的外泌体miRNA可能在癌症的发生和发展中具有重要作用，并且是早期诊断和预后监测的潜在生物标志物。然而，外泌体 miRNA 的简单、灵敏和无标记检测仍然具有挑战性。在此，据报道基于聚合物分选的高纯度半导体碳纳米管 (CNT) 膜的超灵敏、无标记且稳定的场效应晶体管 (FET) 生物传感器可检测外泌体 miRNA。与传统的 CNT FET 不同，CNT FET 生物传感器采用浮栅结构，使用超薄 Y ₂ O ₃作为绝缘层，并在 Y ₂ O ₃上组装 Au 纳米粒子 (AuNPs)作为锚定探针分子的接头。将硫醇化寡核苷酸探针固定在传感区域的 AuNP 表面，然后通过监测固定的 DNA 探针与目标 miRNA 杂交前后的电流变化来检测 miRNA21。该方法实现了高灵敏度（LOD：0.87 aM）和高特异性。此外，FET生物传感器用于测试临床血浆样本，显示健康人和乳腺癌患者之间存在显着差异。CNT FET 生物传感器显示了在乳腺癌临床诊断中的潜在应用。

更新日期：2021-11-23

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11