Extra virgin olive oil (EVOO) is largely used in Mediterranean diet, and it is also worldwide apprised not only for its organoleptic properties but also for its healthy effects mainly attributed to the presence of several naturally occurring phenolic and polyphenolic compounds (bio-phenols). These compounds are characterized by the presence of multiple phenolic groups in more or less complex structures. Their content is fundamental in defining the healthy qualities of EVOO and consequently the analytical methods for their characterization and quantification are of current interest. Traditionally their determination has been conducted using a colorimetric assay based on the reaction of Folin-Ciocalteu (FC) reagent with the functional hydroxy groups of phenolic compounds. Identification and quantification of the bio-phenols in olive oils requires certainly more performing analytical methods. Chromatographic separation is now commonly achieved by HPLC, coupled with spectrometric devices as UV, FID, and MS. This last approach constitutes an actual cutting-edge application for bio-phenol determination in complex matrices as olive oils, mostly on the light of the development of mass analyzers and the achievement of high resolution and accurate mass measurement in more affordable instrument configurations. After a short survey of some rugged techniques used for bio-phenols determination, in this review have been described the most recent mass spectrometry-based methods, adopted for the analysis of the bio-phenols in EVOOs. In particular, the sample handling and the results of HPLC coupled with low- and high-resolution MS and MS/MS analyzers, of ion mobility mass spectrometry and ambient mass spectrometry have been reported and discussed.

中文翻译：

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橄榄油中生物酚的测定：最近的质谱法

特级初榨橄榄油 (EVOO) 主要用于地中海饮食，它不仅因其感官特性而且因其含有多种天然存在的酚类和多酚类化合物（生物酚）而具有健康功效，因此在全世界范围内广为人知. 这些化合物的特征是在或多或少复杂的结构中存在多个酚基。它们的内容对于定义 EVOO 的健康品质至关重要，因此当前对它们的表征和量化分析方法很感兴趣。传统上，它们的测定是使用基于 Folin-Ciocalteu (FC) 试剂与酚类化合物的官能羟基的反应的比色法进行的。橄榄油中生物酚的鉴定和定量当然需要更高效的分析方法。色谱分离现在通常通过 HPLC 与光谱仪如 UV、FID 和 MS 相结合来实现。最后一种方法构成了橄榄油等复杂基质中生物酚测定的实际前沿应用，主要是根据质量分析仪的发展以及在更实惠的仪器配置中实现高分辨率和精确质量测量。在对用于生物酚测定的一些坚固技术进行简短调查后，本综述描述了用于分析 EVOO 中生物酚的最新基于质谱的方法。尤其，