Screening and development of industrial process for enzymatic delignification is an option for those industries who want to minimize acids and chlorine containing bleaches, thus minimizing the impact on environment. For commercial applications, it is important to screen fungi producing lignin modifying enzymes (LMEs) and adapting them to industrial media for submerged fermentations. Using functional plate assays and a full factorial design (FFD) approach, an optimal media composition for the highest expression of these LMEs has been achieved in this study. Two fungal strains (Trametes versicolor and Pleurotus ostreatus) were selected for evaluation. SDS PAGE and native gel analysis confirmed the results of screening and submerged culture media optimizations. When rice straw was added as inducer, secretion of additional enzymes other than laccases such as manganese peroxidase (MnP), lignin peroxidases (LiP), and versatile peroxidase (VP) in the cultures has been observed. This paper also demonstrates an improved process of enzymatic pretreatment resulting from a washing step introduced before saccharification (Scheme. 1). Hence, the current study proposes that removal of LMEs by washing helps in improvement of cellulase/hemicellulose depolymerization efficiency.

酶促脱木素工业过程的筛选和开发是那些希望尽量减少含酸和含氯漂白剂的行业的选择，从而最大限度地减少对环境的影响。对于商业应用，重要的是筛选产生木质素修饰酶 (LME) 的真菌，并将它们应用于工业培养基以进行深层发酵。在本研究中，使用功能板测定和全因子设计 (FFD) 方法，实现了这些 LME 最高表达的最佳培养基组成。两种真菌菌株（Trametes versicolor和Pleurotus ostreatus) 被选中进行评估。SDS PAGE 和天然凝胶分析证实了筛选和浸没培养基优化的结果。当添加稻草作为诱导剂时，在培养物中观察到除漆酶外的其他酶的分泌，如锰过氧化物酶 (MnP)、木质素过氧化物酶 (LiP) 和多功能过氧化物酶 (VP)。该论文还展示了一种改进的酶预处理过程，该过程由糖化之前引入的洗涤步骤产生（方案 1）。因此，目前的研究提出通过洗涤去除 LME 有助于提高纤维素酶/半纤维素解聚效率。