Rapid SARS-CoV-2 Nucleic Acid Testing and Pooled Assay by Tetrahedral DNA Nanostructure Transistor,Nano Letters

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Rapid SARS-CoV-2 Nucleic Acid Testing and Pooled Assay by Tetrahedral DNA Nanostructure Transistor
Nano Letters ( IF 9.6 ) Pub Date : 2021-11-04 , DOI: 10.1021/acs.nanolett.1c02748
Xuejun Wang _{1,

2} , Derong Kong _{1,

2} , Mingquan Guo ₃ , Liqian Wang _{1,

2} , Chenjian Gu ₄ , Changhao Dai _{1,

2} , Yao Wang ₄ , Qunfeng Jiang ₅ , Zhaolin Ai _{1,

2} , Cong Zhang _{1,

2} , Di Qu ₄ , Youhua Xie ₄ , Zhaoqin Zhu ₃ , Yunqi Liu _{2,

6} , Dacheng Wei _{1,

2}

Affiliation

Direct SARS-CoV-2 nucleic acid testing with fast speed and high frequency is crucial for controlling the COVID-19 pandemic. Here, direct testing of SARS-CoV-2 nucleic acid is realized by field-effect transistors (FETs) with an electro-enrichable liquid gate (LG) anchored by tetrahedral DNA nanostructures (TDNs). The applied gate bias electrostatically preconcentrates nucleic acids, while the liquid gate with TDNs provides efficient analyte recognition and signal transduction. The average diagnosis time is ∼80 s, and the limit of detection approaches 1–2 copies in 100 μL of clinical samples without nucleic acid extraction and amplification. As such, TDN-LG FETs solve the dilemma of COVID-19 testing on mass scale that diagnosis accuracy and speed undergo trade-off. In addition, TDN-LG FETs achieve unamplified 10-in-1 pooled nucleic acid testing for the first time, and the results are consistent with PCR. Thus, this technology promises on-site and wide population COVID-19 screening and ensures safe world-reopening.

中文翻译：

通过四面体 DNA 纳米结构晶体管进行快速 SARS-CoV-2 核酸检测和混合检测

快速、高频率的直接 SARS-CoV-2 核酸检测对于控制 COVID-19 大流行至关重要。在这里，SARS-CoV-2 核酸的直接测试是通过场效应晶体管 (FET) 实现的，该晶体管具有由四面体 DNA 纳米结构 (TDN) 锚定的电富集液门 (LG)。施加的门偏压静电预浓缩核酸，而带有 TDN 的液体门提供有效的分析物识别和信号转导。平均诊断时间约为 80 s，检测限在 100 μL 临床样本中接近 1-2 个拷贝，无需核酸提取和扩增。因此，TDN-LG FET 解决了大规模 COVID-19 测试的困境，即诊断准确性和速度需要权衡。此外，TDN-LG FETs首次实现了未扩增的10合1混合核酸检测，结果与PCR一致。因此，这项技术有望在现场和广泛的人群中进行 COVID-19 筛查，并确保安全地重新开放世界。

更新日期：2021-11-24

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