Characterizing the performance of fluorescence microscopy and nonlinear imaging systems is an essential step required for imaging system optimization and quality control during longitudinal experiments. Emerging multimodal nonlinear imaging techniques require a new generation of microscopy calibration targets that are not susceptible to bleaching and can provide a contrast across the multiple modalities. Here, we present a nanodiamond-based calibration target for microscopy, designed for facilitating reproducible measurements at the object plane. The target is designed to support day-to-day instrumentation development efforts in microscopy laboratories. The images of a phantom contain information about the imaging performance of a microscopy system across multiple spectral windows and modalities. Since fluorescent nanodiamonds are not prone to bleaching, the proposed imaging target can serve as a standard, shelf-stable sample to provide rapid reference measurements for ensuring consistent performance of microscopy systems in microscopy laboratories and imaging facilities.

中文翻译：

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用于表征非线性显微镜系统的荧光纳米金刚石

表征荧光显微镜和非线性成像系统的性能是纵向实验期间成像系统优化和质量控制所需的重要步骤。新兴的多模式非线性成像技术需要新一代的显微镜校准目标，这些目标不易漂白，并且可以提供多种模式的对比。在这里，我们提出了一种基于纳米金刚石的显微镜校准目标，旨在促进物平面上的可重复测量。该目标旨在支持显微镜实验室的日常仪器开发工作。幻影的图像包含有关显微镜系统跨多个光谱窗口和模式的成像性能的信息。