Garden asparagus (Asparagus officinalis L.) is a diploid (2n = 2x = 20), perennial and dioecious species belonging to Asparagus subgenus and worldwide cultivated as a vegetable crop. A narrow genetic base has been pointed out for the current cultivars. Crop wild related species (CWR) could be a valuable genetic resource in this crop but they have been underused up to now. To investigate the phylogenetic relationships between CWR asparagus species from different origin and A. officinalis L., 12 EST-SSR markers were used to assess the genetic variability of 20 accessions. These accessions belong to 10 Asparagus spp. from Asparagus subgenus including wild and naturalized A. officinalis L. (2x, 4x, 8x, 10x) and CWR species with European (A. tenuifolius Lam. (2x), A. pseudoscaber Grec. (6x), A. macrorrhizus Pedrol & al. (12x), A. prostratus Dumort (4x), A. brachyphyllus Turcz. (6x), A. maritimus (L.) Mill. (6x)) and Asian distribution (A. verticillatus L. (2x), A. persicus Baker (2x), A. breslerianus Schult. & Schult. (8x)). A. albus L. (2x) from the Protasparagus subgenus was used as outgroup. As a result, a total of 248 alleles were obtained and specific alleles of accessions were detected among them. After cluster analysis the accessions did not group by their geographical origin. All wild polyploid accessions with European and Asian distributions were grouped together with A. officinalis L. Hence, that cluster could be considered as the ‘officinalis group’ suggesting a monophyletic origin. The diploid accessions of A. verticillatus L. and A. persicus Baker clustered together and were the most genetically distant respect to ‘officinalis group’. The results obtained in this study may provide useful information to design new crosses among accessions aimed to develop new asparagus germplasm or pre-breeding populations.

花园芦笋 ( Asparagus officinalis L.) 是一种二倍体 (2n = 2x = 20)，属于芦笋亚属的多年生和雌雄异株物种，在世界范围内作为蔬菜作物种植。已指出当前栽培品种的遗传基础狭窄。作物野生相关物种 (CWR) 可能是该作物的宝贵遗传资源，但迄今为止尚未得到充分利用。为了研究不同来源的 CWR 芦笋物种与A. officinalis L.之间的系统发育关系，使用 12 个 EST-SSR 标记来评估 20 个种质的遗传变异性。这些种质属于 10种芦笋。来自芦笋亚属，包括野生和归化的A. officinalisL. (2x, 4x, 8x, 10x) 和 CWR 物种与欧洲 ( A. tenuifolius Lam. (2x), A. pseudoscaber Grec. (6x), A. macrorrhizus Pedrol & al. (12x) , A. prostratus Dumort (4x)、A. brachyphyllus Turcz. (6x)、A. maritimus (L.) Mill. (6x)) 和亚洲分布 ( A. verticillatus L. (2x) 、A. persicus Baker (2x)、A. breslerianus Schult. & Schult. (8x))。A. albus L. (2x) 来自Protasparagus亚属用作外群。结果共获得248个等位基因，并在其中检测到种质的特定等位基因。聚类分析后，这些种质没有按其地理来源分组。所有具有欧洲和亚洲分布的野生多倍体种质都与A. officinalis L归为一组。因此，该集群可被视为表明单系起源的“厚朴组”。A. verticillatus L. 和A. persicus Baker的二倍体种质聚集在一起，是与 ' officinalis遗传距离最远的尊重团体'。本研究中获得的结果可能为设计旨在开发新芦笋种质或预育种群的种质之间的新杂交提供有用的信息。