The role of intracellular pH (pHi) of lymphocytes in the control of the magnitude of immune response is unknown. We found that low pHi induces apoptosis of proliferating lymphocytes, whereas high pHi promotes their survival. In the ovalbumin sensitization and challenge model, energy metabolism is a major mechanism for regulating pHi. TCA cycle using carbohydrates lowers whereas glutaminolysis or aerobic glycolysis increases pHi. Proliferation powered by high mitochondrial membrane potentials (MMPs) in lymphocytes of low but not high pHi causes apoptosis. After antigenic challenge, lymphocytes of high pHi gradually increase and assume a positive relation between pHi and MMPs while lymphocytes of low pHi and an inverse relation between pHi and MMPs diminish. This change is at least partly dependent on glutaminolysis and aerobic glycolysis.

中文翻译：

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细胞内 pH 值将能量代谢与淋巴细胞死亡和增殖联系起来

淋巴细胞的细胞内 pH (pHi) 在控制免疫反应强度中的作用尚不清楚。我们发现低 pHi 会诱导增殖淋巴细胞凋亡，而高 pHi 会促进其存活。在卵清蛋白致敏和激发模型中，能量代谢是调节 pHi 的主要机制。使用碳水化合物的 TCA 循环会降低 pHi，而谷氨酰胺分解或有氧糖酵解会增加 pHi。在低 pHi 而非高 pHi 的淋巴细胞中，由高线粒体膜电位 (MMP) 驱动的增殖会导致细胞凋亡。抗原攻击后，高pHi淋巴细胞逐渐增多，pHi与MMPs呈正相关，而低pHi淋巴细胞则逐渐减少，pHi与MMPs呈负相关。这种变化至少部分依赖于谷氨酰胺分解和有氧糖酵解。