The SorC/DeoR family is a large family of bacterial transcription regulators that are involved in the control of carbohydrate metabolism and quorum sensing. To understand the structural basis of DNA recognition, structural studies of two functionally characterized SorC/DeoR family members from Bacillus subtilis were performed: the deoxyribonucleoside regulator bsDeoR and the central glycolytic genes regulator bsCggR. Each selected protein represents one of the subgroups that are recognized within the family. Crystal structures were determined of the N-terminal DNA-binding domains of bsDeoR and bsCggR in complex with DNA duplexes representing the minimal operator sequence at resolutions of 2.3 and 2.1 Å, respectively. While bsDeoR_DBD contains a homeodomain-like HTH-type domain, bsCggR_DBD contains a winged helix–turn–helix-type motif. Both proteins form C2-symmetric dimers that recognize two consecutive major grooves, and the protein–DNA interactions have been analyzed in detail. The crystal structures were used to model the interactions of the proteins with the full DNA operators, and a common mode of DNA recognition is proposed that is most likely to be shared by other members of the SorC/DeoR family.

中文翻译：

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SorC/DeoR 家族的细菌转录调节因子对 DNA 识别的结构洞察

SorC/DeoR 家族是一大类细菌转录调节因子，参与控制碳水化合物代谢和群体感应。为了了解 DNA 识别的结构基础，对来自枯草芽孢杆菌的两个功能特征 SorC/DeoR 家族成员进行了结构研究：脱氧核糖核苷调节剂bs DeoR 和中心糖酵解基因调节剂bs CggR。每个选定的蛋白质代表该家族中认可的亚组之一。晶体结构由bs DeoR 和bs的 N 端 DNA 结合结构域确定CggR 与代表最小算子序列的 DNA 双链体复合，分辨率分别为 2.3 和 2.1 Å。虽然bs DeoR _DBD包含类似同源域的 HTH 型结构域，但bs CggR _DBD包含有翼螺旋-转角-螺旋型基序。两种蛋白质都形成识别两个连续大沟的C 2 对称二聚体，并且已经详细分析了蛋白质-DNA 相互作用。晶体结构用于模拟蛋白质与完整 DNA 操作符的相互作用，并提出了一种常见的 DNA 识别模式，该模式最有可能被 SorC/DeoR 家族的其他成员共享。