This review is the tenth update of the original article published in 1999 on the application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

中文翻译：

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通过基质辅助激光解吸/电离质谱法分析碳水化合物和糖复合物：2017-2018 年更新

这篇综述是对 1999 年发表的关于基质辅助激光解吸/电离质谱 (MALDI) 质谱法在碳水化合物和糖缀合物分析中的应用的原始文章的第十次更新，并将文献覆盖范围带到 2018 年底。还包括描述适用于 MALDI 聚糖和糖蛋白分析方法的论文，例如样品制备技术，即使电离方法不是 MALDI。评论第一部分涵盖的主题包括一般方面，例如 MALDI 过程理论、新方法、矩阵、衍生化、MALDI 成像、碎片化和阵列的使用。综述的第二部分致力于各种结构类型的应用，如低聚糖和多糖、糖蛋白、糖脂、糖苷、和生物制药。大多数应用程序都以表格形式呈现。综述的第三部分涵盖了该技术的医学和工业应用、酶反应研究以及化学合成应用。报告的工作显示越来越多地使用离子淌度等组合新技术，并强调了 MALDI 成像在一系列学科中的影响。MALDI 仍然是碳水化合物分析的理想技术，该技术的进步和应用范围继续稳步发展。报告的工作显示越来越多地使用离子淌度等组合新技术，并强调了 MALDI 成像在一系列学科中的影响。MALDI 仍然是碳水化合物分析的理想技术，该技术的进步和应用范围继续稳步发展。报告的工作显示越来越多地使用离子淌度等组合新技术，并强调了 MALDI 成像在一系列学科中的影响。MALDI 仍然是碳水化合物分析的理想技术，该技术的进步和应用范围继续稳步发展。