Strigolactones are carotenoid-derived phytohormones that impact plant growth and development in diverse ways. However, the roles of strigolactones in the responses to temperature stresses are largely unknown. Here, we demonstrated that strigolactone biosynthesis is induced in tomato (Solanum lycopersicum) by heat and cold stresses. Compromised strigolactone biosynthesis or signaling negatively affected heat and cold tolerance, while application of the synthetic strigolactone analog GR245DS enhanced heat and cold tolerance. Strigolactone-mediated heat and cold tolerance was associated with the induction of abscisic acid (ABA), heat shock protein 70 (HSP70) accumulation, C-REPEAT BINDING FACTOR 1 (CBF1) transcription, and antioxidant enzyme activity. Importantly, a deficiency in ABA biosynthesis compromised the GR245DS effects on heat and cold stresses and abolished the GR245DS-induced transcription of HSP70, CBF1, and antioxidant-related genes. These results support that strigolactones positively regulate tomato heat and cold tolerance and that they do so at least partially by the induction of CBFs and HSPs and the antioxidant response in an ABA-dependent manner.

中文翻译：

---

独脚金内酯积极调节番茄脱落酸依赖性耐热性和耐冷性

独脚金内酯是类胡萝卜素衍生的植物激素，以多种方式影响植物的生长和发育。然而，独脚金内酯在温度应激响应中的作用很大程度上未知。在这里，我们证明了独脚金内酯生物合成是在番茄中诱导的（番茄）通过热应力和冷应力。独脚金内酯生物合成或信号传导受损会对耐热性和耐冷性产生负面影响，而合成独脚金内酯类似物 GR24 的应用5DS增强耐热性和耐寒性。独脚金内酯介导的耐热性和耐冷性与诱导脱落酸 (ABA)、热休克蛋白 70 (HSP70) 积累、C-重复结合因子 1（CBF1）转录和抗氧化酶活性。重要的是，ABA 生物合成的缺陷损害了 GR245DS对热应力和冷应力的影响并废除了 GR245DS- 诱导转录热休克蛋白70,CBF1和抗氧化相关基因。这些结果支持独脚金内酯积极调节番茄的耐热性和耐冷性，并且它们至少部分通过诱导 CBF 和 HSP 以及以 ABA 依赖性方式的抗氧化反应来实现这一点。