The animals from bycatch of the shrimp fisheries can be a source of natural products and bioactive compounds. Thus, the present study aimed to evaluate the bioactivity of protein hydrolysates prepared from the two most abundant crabs from the bycatch of shrimp fisheries in Brazil (Callinectes ornatus and Hepatus pudibundus). Samples of C. ornatus and H. pudibundus were collected in the region of Ubatuba, State of São Paulo, Brazil. Muscles with small pieces of exoskeleton of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity was analyzed used three methods: DPPH, sulfhydryl groups, and peroxyl radicals. Additionally, the cytotoxicity of the hydrolysates was investigated using pre-osteoblasts cells. The results showed that the degree of hydrolysis (DH) of H. pudibundus was superior to DH of C. ornatus using both enzymes and was higher when using the enzyme Alcalase 2.4 L® (32.0% ± 1.9). The analysis suggested that the hydrolysates have antioxidant activity. Besides that, no cytotoxic effect was observed on cell viability. Thus, protein hydrolysates of C. ornatus and H. pudibundus have bioactivity, which add value to these bycatch species and suggests their potential use as nutraceutical ingredient in the food industry.

Graphic Abstract

中文翻译：

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从最丰富的甲壳动物兼捕物中获得的蛋白质水解物的生物活性

捕虾业兼捕的动物可以成为天然产物和生物活性化合物的来源。因此，本研究旨在评估由巴西捕虾业兼捕的两种最丰富的螃蟹（Callinectes ornatus和Hepatus pudibundus）制备的蛋白质水解物的生物活性。C. ornatus和H. pudibundus的样本收集于巴西圣保罗州乌巴图巴地区。使用两种酶 Alcalase 2.4 L® 或 Protamex® 水解具有两个物种小块外骨骼的肌肉。使用三种方法分析体外抗氧化能力：DPPH、巯基和过氧自由基。此外，使用前成骨细胞研究了水解产物的细胞毒性。结果表明，使用这两种酶的H. pudibundus的水解度 (DH)优于C. ornatus的 DH，而使用 Alcalase 2.4 L® 酶的水解度 (32.0% ± 1.9) 更高。分析表明水解产物具有抗氧化活性。除此之外，没有观察到对细胞活力的细胞毒性作用。因此， C. ornatus的蛋白质水解物和H. pudibundus具有生物活性，这为这些兼捕物种增加了价值，并暗示了它们在食品工业中作为营养成分的潜在用途。

图形摘要