This work explores the effect of High Pressure (HP) treatment on the proteolytic activity and autolytic capacity of Saccharomyces cerevisiae suspensions, based on yeast extract production. Cellular suspensions were treated at 200–600 MPa for 0–120 min and the activity of vacuolar proteases was measured during autolysis. Moreover, the autolytic capacity of yeast was determined based on the physicochemical characteristics of the produced yeast extract. At pressures of 200 and 400 MPa, proteolytic activity was enhanced up to 160%, after 40 and 10 min of HP treatment, respectively, leading to significantly accelerated autolysis, in combination with cellular permeabilization achieved with HP treatment. However, at 600 MPa, despite the greater cell permeabilization, proteolytic enzymes were gradually inactivated (total inactivation after 15 min of HP treatment), leading to inhibition of autolysis. These results highlight HP as an effective process to enhance endogenous proteolytic activity and thus accelerate yeast extract production.

这项工作探讨了高压 (HP) 处理对Saccharomyces cerevisiae的蛋白水解活性和自溶能力的影响悬浮液，基于酵母提取物的生产。细胞悬液在 200-600 MPa 下处理 0-120 分钟，并在自溶过程中测量液泡蛋白酶的活性。此外，酵母的自溶能力是根据生产的酵母提取物的理化特性确定的。在 200 和 400 MPa 的压力下，HP 处理 40 分钟和 10 分钟后，蛋白水解活性分别增强了 160%，从而显着加速了自溶，结合 HP 处理实现的细胞透化。然而，在 600 MPa 时，尽管细胞通透性更强，但蛋白水解酶逐渐失活（HP 处理 15 分钟后完全失活），从而抑制自溶。