No information is available regarding the utilization of iron (Fe) from different Fe sources at a target tissue level. To detect differences in Fe metabolic utilization among Fe sources, the effect of intravenously injected Fe on growth performance, hematological indices, tissue Fe concentrations and Fe-containing enzyme activities and gene expressions of Fe-containing enzymes or protein in broilers was investigated. On d 22 post-hatching, a total of 432 male chickens were randomly allotted to 1 of 9 treatments in a completely randomized design. Chickens were injected with either a 0.9% (wt/vol) NaCl solution (control) or a 0.9% NaCl solution supplemented with Fe sulphate or 1 of 3 organic Fe sources. The 3 organic Fe sources were Fe chelates with weak (Fe-MetW), moderate (Fe-ProtM) or extremely strong (Fe-ProtES) chelation strength. The 2 Fe dosages were calculated according to the Fe absorbabilities of 10% and 20% every 2 d for a duration of 20 d. Iron injection did not affect (P > 0.05) ADFI, ADG or FCR during either 1 to 10 d or 11 to 20 d after injections. Hematocrit and Fe concentrations in the liver and kidney on d 10 after Fe injections, and Fe concentrations in the liver or pancreas and ferritin heavy-chain (FTH1) protein expression level in the liver or spleen on d 20 after Fe injections increased (P ≤ 0.05) as injected Fe dosages increased. When the injected Fe level was high at 20% Fe absorbability, the chickens injected with Fe-ProtES had lower (P < 0.001) liver or kidney Fe concentrations and spleen FTH1 protein levels than those injected with Fe-MetW or Fe-ProtM on d 20 after injections. And they had lower (P < 0.05) liver cytochrome C oxidase mRNA levels on d 20 after injections than those injected with Fe-MetW or Fe sulphate. The results from this study indicate that intravenously injected Fe from Fe-ProtES was the least utilizable and functioned in the sensitive target tissue less effectively than Fe from Fe sulfate, Fe-MetW or Fe-ProtM.

没有关于在目标组织水平上利用来自不同铁源的铁 (Fe) 的信息。为了检测铁源之间铁代谢利用的差异，研究了静脉注射铁对肉鸡生长性能、血液学指标、组织铁浓度和含铁酶活性以及含铁酶或蛋白质基因表达的影响。在孵化后第 22 天，共有 432 只雄性鸡被随机分配到完全随机设计的 9 个处理中的 1 个。给鸡注射 0.9% (wt/vol) NaCl 溶液（对照）或补充有硫酸铁或 3 种有机铁源中的 1 种的 0.9% NaCl 溶液。3 种有机铁源是具有弱 (Fe-MetW)、中等 (Fe-ProtM) 或极强 (Fe-ProtES) 螯合强度的铁螯合物。2 个 Fe 剂量是根据每 2 天 10% 和 20% 的 Fe 吸收率计算的，持续 20 天。铁剂注射不影响（P  > 0.05) 注射后 1 至 10 天或 11 至 20 天期间的 ADFI、ADG 或 FCR。Fe注射后第10天肝脏和肾脏中的血细胞比容和Fe浓度，注射Fe后第20天肝脏或胰腺中的Fe浓度和肝脏或脾脏中铁蛋白重链（FTH1）蛋白表达水平升高（P  ≤ 0.05）随着注入的铁剂量增加。当注射的 Fe 水平在 20% Fe 吸收率较高时，注射 Fe-ProtES 的鸡在 第注射后20。他们有更低的（P < 0.05) 注射后第 20 天的肝细胞色素 C 氧化酶 mRNA 水平高于注射 Fe-MetW 或硫酸铁的那些。这项研究的结果表明，与来自硫酸铁、Fe-MetW 或 Fe-ProtM 的 Fe 相比，来自 Fe-ProtES 的静脉内注射 Fe 的利用率最低，并且在敏感靶组织中的作用更差。