Silages, as a nutritious and long-term preserved fodder, is achieved through fermentation. However, the constant low temperatures and freeze–thaw event are key factors that affect the quality of silages due to low levels of lactic acid production and undesirable microbial community revitalization. In this study, we attempt to investigate the effects of Lactobacillus plantarum QZW5 on the fermentation characteristics of wheat silage under ensiling conditions at the order of the following temperatures: constant low temperatures (5 °C for 30 days), multigelation (10 ℃ at day and – 10 ℃ at night for 30 days) and room temperature (aerobic/anaerobic, 10 days). Under multigelation condition, Lactobacillus plantarum QZW5 inoculation significantly increased the contents of lactic acid and acetic (P < 0.05) in the silage. The pH, bacterial richness index (Chao) and diversity index (Shannon) of the silage were significantly decreased by Lactobacillus plantarum QZW5 (P < 0.05). In Lactobacillus plantarum QZW5 inoculated silage, the dominant genera were L. plantarum subsp. plantarum. However, those in the control silage were Leuconostoc mesenteroides and Leuconostoc fallax. LAB (lactic acid bacteria) inoculation increased the abundance of desirable Lactobacillus spp. and inhibited the growth of undesirable Escherichia coli, Saccharomyces cerevisiae, and Filamentous fungi, among others in the silage. Therefore, inoculation of L. plantarum QZW5 during ensiling could stimulate favorable fermentation and reconstruct bacterial community for better silage preservation.

中文翻译：

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植物乳杆菌QZW5的特性及多重凝胶对小麦青贮的影响

青贮饲料作为一种营养丰富且可长期保存的饲料，是通过发酵实现的。然而，持续的低温和冻融事件是影响青贮饲料质量的关键因素，因为乳酸产量低和微生物群落不受欢迎。在本研究中，我们试图研究植物乳杆菌 QZW5 在以下温度顺序的青贮条件下对小麦青贮发酵特性的影响：恒定低温（5 °C 持续 30 天）、多凝胶（每天 10 ℃） – 夜间 10 ℃ 30 天）和室温（好氧/厌氧，10 天）。在多凝胶条件下，接种植物乳杆菌QZW5显着提高了青贮饲料中乳酸和乙酸的含量（P < 0.05）。pH 值，植物乳杆菌QZW5显着降低青贮饲料的细菌丰富度指数（Chao）和多样性指数（Shannon）（P < 0.05）。在植物乳杆菌 QZW5 接种的青贮饲料中，优势属是植物乳杆菌亚种。植物园。然而，那些在对照青贮饲料中的是肠系膜明串珠菌和秋串明串珠菌。LAB（乳酸菌）接种增加了理想乳杆菌属的丰度。并抑制青贮饲料中不良大肠杆菌、酿酒酵母和丝状真菌等的生长。因此，青贮期间接种 L. plantarum QZW5 可以促进有利的发酵并重建细菌群落，从而更好地保存青贮。对照青贮饲料中的那些是肠系膜明串珠菌和秋串明串珠菌。LAB（乳酸菌）接种增加了理想乳杆菌属的丰度。并抑制青贮饲料中不良大肠杆菌、酿酒酵母和丝状真菌等的生长。因此，青贮期间接种 L. plantarum QZW5 可以促进有利的发酵并重建细菌群落，从而更好地保存青贮饲料。对照青贮饲料中的那些是肠系膜明串珠菌和秋串明串珠菌。LAB（乳酸菌）接种增加了理想乳杆菌属的丰度。并抑制青贮饲料中不良大肠杆菌、酿酒酵母和丝状真菌等的生长。因此，青贮期间接种 L. plantarum QZW5 可以促进有利的发酵并重建细菌群落，从而更好地保存青贮。