This study describes a promising method for tetraploidy induction in the in vitro grown diploid shoots of the cardiotonic herb Digitalis lanata by colchicine treatment to obtain a higher content of the cardiac glycosides’ digoxin and digitoxin. The best frequency of tetraploids (33 %) induction was observed with 0.5 % colchicine treatment for 8 h. The content of digoxin and digitoxin in the tetraploids increased by 1.61-fold and 1.73-fold over the control diploids respectively. The higher cardiotonic glycoside production in the tetraploid plants of D. lanata have the potential to satisfy the increasing demand of superior plant material for pharmaceutical industries.

本研究描述了一种通过秋水仙碱处理体外生长的强心草本洋地黄二倍体枝条的四倍体诱导方法，以获得更高含量的强心苷地高辛和洋地黄毒苷。用 0.5% 秋水仙碱处理 8 小时观察到四倍体 (33%) 诱导的最佳频率。四倍体中地高辛和洋地黄毒苷的含量分别比对照二倍体增加了1.61倍和1.73倍。D. lanata的四倍体植物中较高的强心苷产量有可能满足制药行业对优质植物材料日益增长的需求。