A short lifespan and increased consumption patterns make e-waste the world's fastest-growing waste stream. Computers are one of the most significant parts of e-waste. Recycling of e-waste has been introduced as the main solution to deal with environmental problems and to save natural mines. This research aims to investigate the bioleaching of Cu, Ni, and Al from computer printed circuit boards (CPCBs) using Penicillium simplicissimum. The adaptation phase began at 1 g/l CPCBs powder with 10⁷ spores and final pulp density was reached at 30 g/l. The most effective parameters including pulp density, initial pH, and the sucrose concentration were optimized to achieve maximum simultaneous leaching efficiency of Cu, Ni, and Al. The results showed the main mechanism of Al and Ni leaching was acidolysis, while complexoysis was the main mechanism for leaching of Cu. The optimal conditions of 60 g/l sucrose concentration, 16 g/l pulp density, and initial pH 6 led to leaching of 100% Cu, 70% Ni, and 98% Al. Additionally, the initial CPCBs sample and the bioleaching residue were analyzed using XRD, HPLC, SEM, and FTIR. The HPLC results confirmed that gluconic and oxalic acids were the main metabolites produced by P. simplicissimum. The SEM micrograph revealed the effectiveness of the bioleaching process in metal leaching. FTIR spectra validated conversion of the molecular structure to simpler materials, as well as the presence of HIPS, PC, and PPO in CPCBs waste powder.

较短的使用寿命和不断增加的消费模式使电子垃圾成为世界上增长最快的垃圾流。计算机是电子垃圾中最重要的部分之一。电子废物回收已被引入作为处理环境问题和拯救天然矿山的主要解决方案。本研究旨在研究使用简单青霉从计算机印刷电路板 (CPCB) 中生物浸出铜、镍和铝。适应阶段开始于 1 g/l CPCBs 粉末和 10 ⁷孢子和最终纸浆密度达到 30 克/升。优化了最有效的参数，包括纸浆密度、初始 pH 值和蔗糖浓度，以实现 Cu、Ni 和 Al 的最大同时浸出效率。结果表明，Al和Ni浸出的主要机理是酸解，而络合是Cu浸出的主要机理。60 g/l 蔗糖浓度、16 g/l 纸浆密度和初始 pH 值 6 的最佳条件导致 100% Cu、70% Ni 和 98% Al 浸出。此外，使用 XRD、HPLC、SEM 和 FTIR 分析了初始 CPCB 样品和生物浸出残留物。HPLC结果证实葡糖酸和草酸是P. simplicissimum产生的主要代谢物. SEM 显微照片揭示了生物浸出工艺在金属浸出中的有效性。FTIR 光谱验证了分子结构向更简单材料的转化，以及 CPCB 废粉中 HIPS、PC 和 PPO 的存在。