Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation,FEBS Letters

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Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation
FEBS Letters ( IF 3.0 ) Pub Date : 2021-10-21 , DOI: 10.1002/1873-3468.14211
Jun-Song Chen ₁ , Christine M Jones ₁ , Maya G Igarashi ₁ , Liping Ren ₁ , Alyssa E Johnson ₁ , Kathleen L Gould ₁

Affiliation

The timing of cytokinesis relative to other mitotic events in the fission yeast Schizosaccharomyces pombe is controlled by the septation initiation network (SIN). During a mitotic checkpoint, the SIN is inhibited by the E3 ubiquitin ligase Dma1 to prevent chromosome mis-segregation. Dma1 dynamically localizes to spindle pole bodies (SPBs) and the contractile ring (CR) during mitosis, though its role at the CR is unknown. Here, we examined whether Dma1 phosphorylation affects its localization or function. We found that preventing Dma1 phosphorylation by substituting the six phosphosites with alanines diminished its CR localization but did not affect its mitotic checkpoint function. These studies reinforce the conclusion that Dma1 localization to the SPB is key to its role in the mitotic checkpoint.

中文翻译：

泛素连接酶 Dma1 在裂殖酵母收缩环上的定位受到磷酸化的调节

裂殖酵母裂殖酵母中胞质分裂相对于其他有丝分裂事件的时间由分隔起始网络（SIN）控制。在有丝分裂检查点期间，SIN 被 E3 泛素连接酶 Dma1 抑制，以防止染色体错误分离。 Dma1 在有丝分裂过程中动态定位于纺锤体极体 (SPB) 和收缩环 (CR)，但其在 CR 中的作用尚不清楚。在这里，我们检查了 Dma1 磷酸化是否影响其定位或功能。我们发现，通过用丙氨酸取代六个磷酸位点来防止 Dma1 磷酸化会减少其 CR 定位，但不会影响其有丝分裂检查点功能。这些研究强化了这样的结论：Dma1 定位于 SPB 是其在有丝分裂检查点中发挥作用的关键。

更新日期：2021-11-22

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