当前位置:
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
ThpacC Acts as a Positive Regulator of Homodimericin A Biosynthesis and Antifungal Activities of Trichoderma harzianum 3.9236
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-22 , DOI: 10.1021/acs.jafc.1c04330 Mengyue Wu 1, 2 , Huiling Wei 1 , Ke Ma 2 , Peiqi Cui 1 , Shaozhou Zhu 1 , Daowan Lai 3 , Jinwei Ren 4 , Wenzhao Wang 4 , Aili Fan 1, 2 , Wenhan Lin 2 , Haijia Su 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-22 , DOI: 10.1021/acs.jafc.1c04330 Mengyue Wu 1, 2 , Huiling Wei 1 , Ke Ma 2 , Peiqi Cui 1 , Shaozhou Zhu 1 , Daowan Lai 3 , Jinwei Ren 4 , Wenzhao Wang 4 , Aili Fan 1, 2 , Wenhan Lin 2 , Haijia Su 1
Affiliation
The Pal/Rim pathway and its key transcription factor PacC play important roles in fungal adaptation to ambient pH regarding growth, secondary metabolism, and virulence. However, the effect of PacC on the secondary metabolism of the important biocontrol fungus Trichoderma harzianum remains elusive. To answer this question, ThpacC deletion (KO-ThpacC) and overexpression (OE-ThpacC) mutants of T. harzianum 3.9236 were constructed. Transcriptomic analysis of T. harzianum and KO-ThpacC suggested that ThpacC acted as both a positive and a negative regulator for secondary metabolite (SM) production. Further investigation revealed that deletion of ThpacC abolished homodimericin A and 8-epi-homodimericin A production. Moreover, ThpacC plays a role in the antagonism of T. harzianum against Sclerotinia sclerotiorum. 8-epi-Homodimericin A demonstrated moderate inhibitory activity against S. sclerotiorum. Our results contribute to a deeper understanding of the ThpacC function on SM production and the antifungal activity of T. harzianum.
中文翻译:
ThpacC 是哈茨木霉 3.9236 同源二聚蛋白 A 生物合成和抗真菌活性的正调节剂
Pal/Rim 通路及其关键转录因子 PacC 在真菌对环境 pH 值的生长、次生代谢和毒力方面的适应中发挥重要作用。然而,PacC 对重要生物防治真菌哈茨木霉次级代谢的影响仍然难以捉摸。为了回答这个问题,构建了哈茨木霉3.9236 的ThpacC缺失(KO-ThpacC)和过表达(OE-ThpacC)突变体。T. harzianum和KO-ThpacC 的转录组学分析表明,ThpacC 对次级代谢物 (SM) 的产生起到了积极和消极的调节作用。进一步调查发现,删除ThpacC消除了同源二聚蛋白A 和 8- epi- homodimericin A 的产生。此外,ThpacC 在哈茨木霉对抗核盘菌的拮抗作用中起作用。8- epi- Homodimericin A 表现出对核盘菌的中等抑制活性。我们的结果有助于更深入地了解 ThpacC 对 SM 生产的功能和哈茨木霉的抗真菌活性。
更新日期:2021-11-03
中文翻译:
ThpacC 是哈茨木霉 3.9236 同源二聚蛋白 A 生物合成和抗真菌活性的正调节剂
Pal/Rim 通路及其关键转录因子 PacC 在真菌对环境 pH 值的生长、次生代谢和毒力方面的适应中发挥重要作用。然而,PacC 对重要生物防治真菌哈茨木霉次级代谢的影响仍然难以捉摸。为了回答这个问题,构建了哈茨木霉3.9236 的ThpacC缺失(KO-ThpacC)和过表达(OE-ThpacC)突变体。T. harzianum和KO-ThpacC 的转录组学分析表明,ThpacC 对次级代谢物 (SM) 的产生起到了积极和消极的调节作用。进一步调查发现,删除ThpacC消除了同源二聚蛋白A 和 8- epi- homodimericin A 的产生。此外,ThpacC 在哈茨木霉对抗核盘菌的拮抗作用中起作用。8- epi- Homodimericin A 表现出对核盘菌的中等抑制活性。我们的结果有助于更深入地了解 ThpacC 对 SM 生产的功能和哈茨木霉的抗真菌活性。