Odorant-Binding Protein 1 Plays a Crucial Role in the Olfactory Response of Bemisia tabaci to R-Curcumene,Journal of Agricultural and Food Chemistry

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Odorant-Binding Protein 1 Plays a Crucial Role in the Olfactory Response of Bemisia tabaci to R-Curcumene
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-20 , DOI: 10.1021/acs.jafc.1c03825
Haixia Zhan ₁ , Youssef Dewer ₂ , Jinping Zhang ₃ , Jiahui Tian ₁ , Du Li ₁ , Cheng Qu ₁ , Zhen Yang ₄ , Fengqi Li ₁ , Chen Luo ₁

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The cultivated tomato Solanum lycopersicum suffered a severe attack by the whitefly Bemisia tabaci (Gennadius), causing damage to leaves by feeding as well as transmitting the tomato yellow leaf curl virus (TYLCV), while the wild tomato S. habrochaites is considerably less appealing to this insect species. It is reported that B. tabaci shows innate avoidance to R-curcumene, which is produced naturally by S. habrochaites. However, the mechanisms involved in the avoidance behavior of B. tabaci in response to this chiral compound are still unclear yet. In this study, the functional and binding characterization of odorant-binding protein 1 of B. tabaci (BtOBP1) were examined in vivo and in vitro against R-curcumene. The obtained results showed that BtOBP1 exhibits specific binding activity to R-curcumene, which acts as repellents to B. tabaci. By using a fluorescence-based binding assay, the difference of binding-affinity for R-curcumene between wild type BtOBP1 and the mutant BtOBP1 to R-curcumene was performed, which resulted in a single amino acid mutation (ASN108 > SER); moreover, BtOBP1-N108 displays significantly decreased binding affinities to R-curcumene. Most interestingly, a knock-down experiment with the BtOBP1 showed that the whitefly responses to R-curcumene are impaired. This study illustrated that BtOBP1 is a crucial protein involved in the perception and discrimination of R-curcumene. Our findings may provide an excellent chance of finding a suitable antagonist of eco-friendly features that can block the perception of chemosensory signals in insects, preventing behaviors like food-finding.

中文翻译：

气味结合蛋白 1 在烟粉虱对 R-姜黄烯的嗅觉反应中起关键作用

栽培番茄Solanum lycopersicum遭受粉虱Bemisia tabaci (Gennadius)的严重攻击，通过取食和传播番茄黄叶卷曲病毒 (TYLCV) 对叶片造成损害，而野生番茄S. habrochaites 对它的吸引力要小得多。这种昆虫种类。据报道，烟粉虱表现出对由S. habrochaites 自然产生的R-姜黄烯的先天回避。然而，烟粉虱响应这种手性化合物的回避行为所涉及的机制尚不清楚。在这项研究中，气味结合蛋白 1 的功能和结合表征烟粉虱(BtOBP1)在体内和体外针对R-姜黄烯进行了检查。所得结果表明，BtOBP1 对R-姜黄烯表现出特异性结合活性，R-姜黄烯可作为烟粉虱的驱避剂。通过使用基于荧光的结合测定，进行了野生型BtOBP1和突变体BtOBP1对R-姜黄烯之间对R-姜黄烯的结合亲和力的差异，导致单个氨基酸突变（ASN108 > SER）；此外，BtOBP1-N108 对R 的结合亲和力显着降低-姜黄素。最有趣的是，BtOBP1 的敲除实验表明粉虱对R-姜黄烯的反应受损。该研究表明，BtOBP1 是一种重要的蛋白质，参与R-姜黄素的感知和辨别。我们的研究结果可能提供了一个极好的机会，可以找到一种合适的环保特征拮抗剂，这些拮抗剂可以阻断昆虫对化学感应信号的感知，从而阻止寻找食物等行为。

更新日期：2021-11-03

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