Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes. Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, N=6) versus hypertensive non-diabetic patients (AH group, N=34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test. In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (P=0.004) and B cells (P=0.025) in the tunica intima, and tunica media (P=0.049, P=0.007, respectively), and fewer plasma cells in the tunica media (P=0.024) and tunica adventitia (P=0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (P=0.025). Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall, and more collagen fibers in tunica adventitia.

中文翻译：

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2型糖尿病患者与高血压患者升主动脉瘤中的炎症细胞。

主动脉瘤较常发生在升主动脉，但很少见于 2 型糖尿病患者。我们的目的是评估无动脉高血压的糖尿病患者（DM2 组，N=6）与高血压非糖尿病患者（AH 组，N=34）的升主动脉瘤壁炎症细胞浸润情况。对于组织学分析，切片用苏木精-伊红和 Movat 五色染色。免疫组化染色用于分析促炎细胞（CD68）和抗炎巨噬细胞（CD163）、T辅助细胞（CD4）和T杀伤细胞（CD8）以及B（CD79a）和浆细胞（CD138）的浸润情况。两组的所有三层动脉瘤。通过ANOVA和Welch检验评估组间差异的统计学显着性。与AH组相比，DM2 组在内膜和中膜（分别为 P=0.049，P=0.007）中促炎巨噬细胞（P=0.004）和 B 细胞（P=0.025）浸润较轻，而在中膜 (P=0.024) 和外膜 (P=0.017)。我们发现血管壁所有三层中 T 辅助细胞、T 杀伤细胞和抗炎巨噬细胞的数量以及胶原蛋白和弹性纤维、基质和平滑肌细胞的数量没有显着差异。除DM2组外膜外膜外，胶原纤维总体较多（P=0.025）。因此，我们得出结论，无高血压的糖尿病患者动脉瘤的组织学结构与无糖尿病的高血压患者几乎相同。