Abstract

Considering the importance of the embryonic stage, we investigated the brain and serum ADA of offspring chronically exposed to low and high frequency electromagnetic field (EMF-LF/HF) during their embryonic period. Male and female rats were randomly selected for mating. After mating, the females were divided into four groups. The first group was exposed to extremely EMF-LF (50 Hz) through a solenoid for 30 minutes per day, the second group was constantly exposed to EMF-HF (900 MHz) via a cell phone, the third group (sham) was placed inside solenoid without any exposure, and the last group (control) was placed in their cage. The brain and serum samples of offspring (6 females and 6 males in each group) were collected four weeks after birth. ADA was measured by a specific enzymatic kit and a spectrophotometer. Exposure to EMF-LF (1.5 mT) was significantly increased the brain ADA activity in both male and female offspring. In addition, long-term HF exposure significantly enhanced serum ADA activity in males in compared with control group but, the brain ADA level was increased significantly in both genders(p < 0.01). The present study revealed alterations in the serum and brain ADA following embryonic exposure to HF and ELF. These alterations depended on gender, frequency and wave intensity.

摘要

考虑到胚胎阶段的重要性，我们研究了在胚胎期长期暴露于低频和高频电磁场 (EMF-LF/HF) 的后代的大脑和血清 ADA。随机选择雄性和雌性大鼠进行交配。交配后，雌性被分成四组。第一组每天通过螺线管暴露于极端 EMF-LF (50 Hz) 30 分钟，第二组通过手机不断暴露于 EMF-HF (900 MHz)，第三组（假）被放置内部电磁阀没有任何暴露，最后一组（对照组）被放置在他们的笼子里。出生后4周收集后代（每组6只雌性和6只雄性）的脑和血清样本。ADA 通过特定的酶促试剂盒和分光光度计测量。暴露于 EMF-LF (1. 5 mT) 显着增加了雄性和雌性后代的大脑 ADA 活动。此外，与对照组相比，长期 HF 暴露显着增强男性血清 ADA 活性，但脑 ADA 水平在两性中均显着升高（p < 0.01）。本研究揭示了胚胎暴露于 HF 和 ELF 后血清和脑 ADA 的变化。这些改变取决于性别、频率和波浪强度。