The potential of MALDI-TOF MS was investigated in terms of its capability to determine the change of exudates of fish muscle with different freeze-thaw cycles (0, 1 and 2), and frozen storage periods. The exudates were collected from dead chilled marine fish species, large yellow croacker (Larimichthys crocea, LC) and freshly slaughtered freshwater fish species, Japanese seabass (Lateolabrax japonicus) to be studied as models. 109 proteins, in which, 32 are extracellular proteins, and 15 are intracellular proteins, were identified by analyzing exudate of LC using MALDI-TOF MS and HPLC-MS/MS. The results show that the present method may be able to determine the change of fish muscle foods in a more sensitive mode than K value indicated quality control. The feasibility of verifying the storage situation of fish sample was performed by analyzing fish samples obtained from the local market. It is promising to estimate the storage situation of fishery products by analyzing their muscle exudates based on the presently developed strategy.

MALDI-TOF MS 的潜力在其确定不同冻融周期（0、1 和 2）和冷冻储存期的鱼肌肉渗出物变化的能力方面进行了研究。渗出液是从冷冻的死海鱼、大黄鱼( Larimichthys crocea , LC) 和新鲜屠宰的淡水鱼日本鲈鱼 ( Lateolabrax japonicus )中收集的，作为模型进行研究。使用MALDI-TOF MS和HPLC-MS/MS分析LC的渗出物，鉴定出109种蛋白质，其中32种为细胞外蛋白质，15种为细胞内蛋白质。结果表明，本方法可能能够以比K更灵敏的模式确定鱼肌肉食物的变化。值表示质量控制。通过分析从当地市场获得的鱼样品，验证了鱼样品储存情况的可行性。基于目前制定的策略，通过分析其肌肉渗出物来估计渔业产品的储存情况是有希望的。