Continuous translation elongation, irrespective of amino acid sequences, is a prerequisite for living organisms to produce their proteomes. However, nascent polypeptide products bear an inherent risk of elongation abortion. For example, negatively charged sequences with occasional intermittent prolines, termed intrinsic ribosome destabilization (IRD) sequences, weaken the translating ribosomal complex, causing certain nascent chain sequences to prematurely terminate translation. Here, we show that most potential IRD sequences in the middle of open reading frames remain cryptic and do not interrupt translation, due to two features of the nascent polypeptide. Firstly, the nascent polypeptide itself spans the exit tunnel, and secondly, its bulky amino acid residues occupy the tunnel entrance region, thereby serving as a bridge and protecting the large and small ribosomal subunits from dissociation. Thus, nascent polypeptide products have an inbuilt ability to ensure elongation continuity.

中文翻译：

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出口通道内的新生多肽稳定核糖体以抵消危险的翻译


无论氨基酸序列如何，连续翻译延伸是活生物体产生蛋白质组的先决条件。然而，新生的多肽产品具有伸长失败的固有风险。例如，带有偶尔间歇性脯氨酸的带负电荷序列，称为内在核糖体不稳定（IRD）序列，会削弱翻译核糖体复合物，导致某些新生链序列过早终止翻译。在这里，我们表明，由于新生多肽的两个特征，开放阅读框中间的大多数潜在 IRD 序列仍然是神秘的，并且不会中断翻译。首先，新生多肽本身跨越出口隧道，其次，其庞大的氨基酸残基占据隧道入口区域，从而充当桥梁并保护大小核糖体亚基免于解离。因此，新生的多肽产物具有确保延伸连续性的内在能力。