Previous researches have been conducted evaluating the volatile compounds of pork. However, data regarding the changes in volatiles and metabolic pathways during pork storage were inadequately investigated. Herein, a headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC/MS) coupled multivariate analysis was proposed for characterizing the profiles of volatile compounds and metabolic pathways during pork storage. A total of 37 metabolites, including aldehydes, ketones, alcohols etc. were successfully identified. Multivariate statistical analysis revealed a substantial variation in metabolite phenotype among samples over the pork storage period, with 12 characteristic metabolites and 5 potential characteristic metabolites screened as biomarkers. Moreover, three metabolomic pathways analysis and transformation between each other (thermal reactions, lipid metabolism and amino acid metabolism) reveals the underlying mechanisms of metabolites change of pork. Therefore, the present study may provide insight into future understanding of the variation in the pork metabolite profiles.

以前的研究已经进行了评估猪肉的挥发性化合物。然而，关于猪肉储存期间挥发物和代谢途径变化的数据没有得到充分研究。在此，提出了顶空固相微萃取-气相色谱-质谱 (HS-SPME-GC/MS) 耦合多变量分析，用于表征猪肉储存过程中挥发性化合物和代谢途径的特征。共成功鉴定出醛、酮、醇等37种代谢物。多变量统计分析显示，猪肉储存期间样品中代谢物表型存在显着差异，筛选出 12 种特征代谢物和 5 种潜在特征代谢物作为生物标志物。而且，三种代谢组学途径分析和相互转化（热反应、脂质代谢和氨基酸代谢）揭示了猪肉代谢物变化的潜在机制。因此，本研究可以为未来对猪肉代谢物谱变化的理解提供见解。