In Korea, genetically modified (GM) canola events derived from eleven single events have been authorized for food and feed, but not for cultivation. Therefore, the development of a rapid and accurate on-site detection method is crucial for the management of these approved GM canola events. In this study, ultrafast polymerase chain reaction (PCR) assays for the event-specific detection of eleven GM canola events were developed. The limit of detection (LOD) on DNA-based and powder-based GM canola samples of each primer set using the ultrafast PCR ranged from 0.1% to 0.01%, while the quantitative analysis of these ultrafast PCR assays, indicated that the correlation coefficient (R²) ranged from 0.98 to 0.9903. These results indicate that the developed assays may have sufficient specificity and LOD capacity to detect the eleven specific GM canola events for the attendant management and monitoring, thus preventing GM canola from contaminating the natural environment.

在韩国，源自 11 个单一事件的转基因 (GM) 双低油菜籽事件已被授权用于食品和饲料，但不能用于种植。因此，开发一种快速准确的现场检测方法对于管理这些获批的转基因油菜事件至关重要。在这项研究中，开发了用于事件特异性检测的 11 种转基因油菜事件的超快聚合酶链反应 (PCR) 分析。使用超快速 PCR 对每个引物组的 DNA 和粉末基转基因油菜样品的检测限 (LOD) 范围为 0.1% 至 0.01%，而这些超快速 PCR 测定的定量分析表明相关系数（ [R ²) 的范围从 0.98 到 0.9903。这些结果表明，开发的检测方法可能具有足够的特异性和 LOD 能力来检测 11 种特定的转基因油菜事件，用于随之而来的管理和监测，从而防止转基因油菜污染自然环境。