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A new potentiometric PVC membrane sensor for the determination of DPPH radical scavenging activity of plant extracts
Food Chemistry ( IF 8.5 ) Pub Date : 2021-10-20 , DOI: 10.1016/j.foodchem.2021.131420
Ömer Isildak 1 , Ilyas Yildiz 1 , Nusret Genc 1
Affiliation  

In this study, a simple, fast, stable and low-cost potentiometric determination method is recommended for the evaluation of 1,1-diphenyl-1-picrylhydrazil radical (DPPH•) scavenging activity, which is one of the antioxidant activity tests. For this purpose, a new type of solid-state-contact potentiometric polyvinyl chloride (PVC) membrane sensor was developed. Gallic acid compound, which is sensitive to the DPPH•, was used as the active ingredient in the membrane structure of the sensor. The potentiometric behavior of the developed DPPH•-selective PVC membrane sensor was characterized. The detection limit of the developed sensor was obtained as 2.3 µg/mL. The sensor can measure at low volumes with its high selectivity. The measurement results obtained were in accordance with the results of the spectroscopic method in the literature. The developed DPPH•-selective PVC membrane sensor was quickly dipped directly into the plant extracts and successfully used in test measurements of DPPH• scavenging activity.



中文翻译:

一种用于测定植物提取物DPPH自由基清除活性的新型电位PVC膜传感器

本研究推荐使用一种简单、快速、稳定、低成本的电位测定方法来评价1,1-二苯基-1-苦基肼自由基(DPPH•)清除活性,这是抗氧化活性测试之一。为此,开发了一种新型固态接触电位聚氯乙烯 (PVC) 膜传感器。对DPPH• 敏感的没食子酸化合物被用作传感器膜结构中的活性成分。对开发的 DPPH• 选择性 PVC 膜传感器的电位行为进行了表征。开发的传感器的检测限为 2.3 µg/mL。该传感器可以以其高选择性进行小体积测量。得到的测量结果与文献中光谱法的结果一致。

更新日期:2021-10-26
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