Consistent exposure to 17β-estradiol through drinking water and food can cause health problems. Although many simple and sensitive fluorescence sensors of 17β-estradiol have been reported, most of them are based on fluorescence quenching test mode working in visible light range, which are inferior in anti-interference ability and quantitative range. Here, we developed a near-infrared (NIR) phosphorescence aptasensor for the detection of 17β-estradiol that has no background fluorescence. The aptasensor was based on Foster resonance energy transfer (FRET) between aptamer conjugated NIR persistent luminescence nanoparticles (PLNPs-Apt) and MoS₂ nanosheets. The 17β-estradiol was quantified by the recovery of PLNPs’ phosphorescence. This assay can detect 17β-estradiol in 0.5 mL samples with the LOD of 0.29 ng mL^-1 and in concentrations of more than three orders of magnitude (from 0.5 ng mL^-1 to 1.2 μg mL^-1). This aptasensor exhibited selectivity for 17β-estradiol and was applicable in complex milk samples.

通过饮用水和食物持续接触 17β-雌二醇会导致健康问题。虽然已经报道了许多简单而灵敏的17β-雌二醇荧光传感器，但大多是基于荧光猝灭测试模式工作在可见光范围内，抗干扰能力和定量范围较差。在这里，我们开发了一种近红外 (NIR) 磷光适体传感器，用于检测没有背景荧光的 17β-雌二醇。适体传感器基于适体结合的 NIR 持久发光纳米粒子 (PLNPs-Apt) 和 MoS ₂纳米片之间的福斯特共振能量转移 (FRET) 。17β-雌二醇通过 PLNPs 磷光的恢复来量化。该测定可检测 0.5 mL 样品中的 17β-雌二醇，LOD 为 0.29 ng mL^-1并且浓度超过三个数量级（从 0.5 ng mL ^-1到 1.2 μg mL ^-1）。该适体传感器对 17β-雌二醇具有选择性，适用于复杂的牛奶样品。