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Electrochemical sensors for the detection of SARS-CoV-2 virus
Chemical Engineering Journal ( IF 13.3 ) Pub Date : 2021-10-19 , DOI: 10.1016/j.cej.2021.132966
Neeraj Kumar 1 , Nagaraj P Shetti 2 , Somanath Jagannath 3 , Tejraj M Aminabhavi 2
Affiliation  

Coronavirus (COVID-19), a deadly pandemic has spread worldwide and created many global health issues. Though methods of its detection are being continuously developed for the early detection and monitoring of COVID-19, still there is need for more novel methods. The presently used methods include rapid antigen tests, serological surveys, reverse transcription-polymerase chain reaction (RT-PCR), artificial intelligence-based techniques, and assays based on sensors/biosensors. Of all these, RT-PCR test has high sensitivity and specificity though it requires more time for testing and need for skilled technicians. Recently, electrochemical sensors have been developed for rapid monitoring and detection of SARS-CoV-2 from the patient’s biological fluid samples. This review covers the recently developed electrochemical sensors that are focused on the detection of viral nucleic acid, immunoglobulin, antigen, and the entire viral particles. In addition, we also compare and assess their detection limits, sensitivities and specificities for the identification and monitoring of COVID-19. Furthermore, this review will address the best practices for the development of electrochemical sensors such as electrode fouling, limit of detection/limit of quantification determination and verification.



中文翻译:


用于检测 SARS-CoV-2 病毒的电化学传感器



冠状病毒(COVID-19)是一种致命的流行病,已在全球范围内传播,并造成了许多全球健康问题。尽管为了早期检测和监测 COVID-19,其检测方法正在不断开发,但仍然需要更多新颖的方法。目前使用的方法包括快速抗原检测、血清学调查、逆转录聚合酶链反应(RT-PCR)、基于人工智能的技术以及基于传感器/生物传感器的测定。其中,RT-PCR检测具有较高的灵敏度和特异性,但检测时间较长,需要熟练的技术人员。最近,电化学传感器被开发出来,用于快速监测和检测患者生物体液样本中的 SARS-CoV-2。本综述涵盖了最近开发的电化学传感器,这些传感器专注于病毒核酸、免疫球蛋白、抗原和整个病毒颗粒的检测。此外,我们还比较和评估了它们在识别和监测 COVID-19 方面的检测限、敏感性和特异性。此外,本次审查还将讨论电化学传感器开发的最佳实践,例如电极污染、检测限/定量限确定和验证。

更新日期:2021-10-20
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